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Quantitative detection of Pythium porphyrae and Pythium chondricola (Oomycota), the causative agents of red rot disease in Pyropia farms in China

文献类型： 外文期刊

作者： Liu, Jie 1 ; Xia, Sudong 1 ; Yang, Huichao 2 ; Mo, Zhaolan 2 ; Li, Jie 2 ; Yan, Yongwei 2 ;

作者机构： 1.Tianjin Agr Univ, Dept Fishery Sci, Tianjin Key Lab Aqua Ecol & Aquaculture, Tianjin 300384, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, State Key Lab Mariculture Biobreeding & Sustainabl, Qingdao 266071, Peoples R China

3.Laoshan Lab, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China

4.Ocean Univ China, Sanya Oceanog Inst, Key Lab Trop Aquat Germplasm Hainan Prov, Sanya 572000, Peoples R China

关键词： Pyropia; Pythium chondricola; Pythium porphyrae; quantification; red rot disease

期刊名称：ALGAE （ 影响因子：2.4； 五年影响因子：2.0 ）

ISSN： 1226-2617

年卷期： 2024 年 39 卷 3 期

页码：

收录情况： SCI

摘要： Red rot disease is one of the notorious algal diseases that threaten the cultivation of Pyropia in China, and two Pythium pathogens, i.e., Pythium porphyrae and P. chondricola, have been reported as causative agents. To monitor the pathogens, a fluorescent quantitative polymerase chain reaction (PCR) method was developed to quantitatively detect their abundance. Using overlapping PCR and pathogen-specific primer pairs, two pathogen-specific fragments were concatenated to construct an internal standard plasmid, which was used for quantification. For zoospores of known numbers, the results showed that this method can detect as less as 100 and 10 zoospores mL-1 in a 200 mL solution for P. porphyrae and P. chondricola, respectively. Using monthly collected seawater at 10 sites in Haizhou Bay, a typical aquaculture farm in China, a significantly higher temperature and a significantly lower salinity were determined in December 2021. P. porphyrae was determined to be more abundant than P. chondricola, though with similar temporal distribution patterns from December 2021 to February 2022. When a red rot disease occurred in December 2021, the two pathogens were significantly more abundant at two infected sub-sites than the uninfected sub-site within both seawater and sediment, though they were all significantly more enriched in sediment than in seawater. The present method provides the capability to quantify and compare the abundance of two pathogens and also has the potential to forecast the occurrence of red rot disease, which is of much significance in managing and controlling the disease.

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