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Cloning and association analysis with cold adaptation of NADH-quinone oxidoreductase 3 subunit gene from common carp Cyprinus carpio

文献类型： 外文期刊

作者： Liang, Liqun 1 ; Chang, Yumei 2 ; Zou, Qingwei 3 ; Lei, Qingquan 1 ;

作者机构： 1.Harbin Univ Sci & Technol, Coll Elect & Elect Engn, Harbin 150080, Peoples R China

2.Chinese Acad Fishery Sci, Heilongjiang River Fisher Res Inst, Harbin 150070, Peoples R China

3.Nankai Univ, Coll Life Sci, Tianjin 300071, Peoples R China

关键词： common carp Cyprinus carpio;NADH-quinone oxidoreductase 3 subunit gene;cloning;cold adaptation

期刊名称：ICPADM 2009: PROCEEDINGS OF THE 9TH INTERNATIONAL CONFERENCE ON PROPERTIES AND APPLICATIONS OF DIELECTRIC MATERIALS, VOLS 1-3

ISSN：

年卷期： 2009 年

页码：

收录情况： SCI

摘要： Genes expressed specifically at cooling temperature were used to design primers and to screen brain full-length cDNA library of common carp (Cyprinus carpio) through two rounds of clonal PCR method. After that, gene 152 was discovered and sequenced, and determined finally as NADH-quinone oxidoreductase 3 subunit gene using homologous searches. This gene was confirmed to play an important role as the force generators for fish in a cooling water environment. In addition, NADH-quinone oxidoreductase 3 subunit gene was associated with cold adaptation of common carp to some extent in oxidation of respiratory chain electron transport system.

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