Quantitative determination of prothioconazole in wheat grain, soybean, and pond water based on a polyclonal antibody
文献类型： 外文期刊
作者： Guo, Zhihui 1 ; Deng, Mingya 3 ; Fang, Wenwen 3 ; Zheng, Kang 3 ; Liao, Min 2 ; Wang, Yulong 4 ; Fang, Qingkui 1 ;
作者机构： 1.Anhui Agr Univ, Key Lab Agriprod Qual & Biosafety, Minist Educ, Hefei 230036, Peoples R China
2.Anhui Agr Univ, Joint Res Ctr Food Nutr & Hlth IHM, Hefei 230036, Peoples R China
3.Anhui Agr Univ, Sch Plant Protect, Anhui Prov Key Lab Integrated Pest Management Crop, Hefei 230036, Peoples R China
4.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr, State Key Lab Breeding Base, Key Lab Food Qual & Safety Jiangsu Prov,Minist Agr, Nanjing 210014, Peoples R China
5.Xinjiang Acad Agr & Reclamat Sci, Food Qual Supervis & Testing Ctr, Minist Agr, Shihezi, Xinjiang, Peoples R China
期刊名称：ANALYTICAL METHODS （ 影响因子：3.1； 五年影响因子：3.0 ）
ISSN： 1759-9660
年卷期： 2024 年
页码：
收录情况： SCI
摘要： Prothioconazole and its metabolite are considered a potential threat to human health and environmental safety. Thus, the development of a sensitive and rapid detection method for prothioconazole is crucial to ensure the safety of agricultural products. In this study, a new hapten of prothioconazole was designed and synthesized, and a selective polyclonal antibody with high affinity against prothioconazole was produced, which was obtained from immunized New Zealand white rabbits. Based on the polyclonal antibody, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and indirect competitive chemiluminescence enzyme immunoassay (ic-CLEIA) were developed for detecting prothioconazole pesticides. Under optimized experimental conditions, the limit of quantification (LOQ) values for ic-CLEIA and ic-ELISA were 1.8 and 10.7 ng mL-1, respectively. The results demonstrated that the sensitivity (LOQ) achieved by ic-CLEIA was more than five times higher compared to that obtained with ic-ELISA. In addition, the recoveries obtained by adding standard prothioconazole to wheat grain, soybean, and pond water samples were in the range of 81.9 to 104.7% for ic-ELISA and 89.0 to 118.0% for ic-CLEIA. Prothioconazole and its metabolite are considered a potential threat to human health and environmental safety.
- 相关文献
作者其他论文 更多>>
-
Using designed polypeptides to develop novel phage-mediated triple antibody sandwich immunoassay for the specific detection of Escherichia coli O157:H7 in milk
作者：Xue, Chang;Ouyang, Qin;Zhang, Cunzheng;Xue, Chang;Fu, Jinmei;Tao, Tingting;Zhu, Shouhu;Han, Bingsong;Wang, Yulong;Ye, Yuhui;Zhang, Cunzheng;Fu, Jinmei;Zhang, Cunzheng;Zhu, Shouhu;Zhang, Cunzheng;Hammock, Bruce D.;Hammock, Bruce D.;Luo, Ruifeng
关键词：Polypeptides; Phage-displayed nanobody; Serotype discrimination; Triple antibody sandwich immunoassay; Escherichia coli O157:H7
-
Development of colorimetric immunochromatographic assay based on different morphologies of gold nanoparticles for the detection of triadimefon
作者：Dong, Sa;Zhang, Yu;Shi, Qiuyun;Zhu, Zixin;Feng, Jianguo;Wang, Yulong;Luo, Ruifeng
关键词：Triadimefon; Immunochromatographic strip; Gold nanoparticles; Gold nanoflowers; Gold nanorods; Monoclonal antibody
-
Minimalist nanobody architecture: A double-edged sword for immunological analysis
作者：Wang, Yulong;Liu, Pengyan;Zhang, Yaodong;Zhang, Cunzheng;Wang, Yulong;Luo, Ruifeng;Yang, Huijuan;Dong, Sa;Hua, Xiude;Hammock, Bruce D.
关键词：Nanobody-based immunoassay; Miniaturized structure; Small size; Bioconjugation chemistry
-
Investigation and insights into the technical strategies for reducing agrochemical inputs in rice farming to enhance agroecosystem resilience and food safety, A case study in China
作者：Zhang, Yaodong;Zhang, Haoran;Zhang, Cunzheng;Zhang, Yaodong;Zhang, Haoran;Chen, Jian;Li, Pan;Wang, Yulong;Liu, Pengyan;Zhang, Cunzheng;Tao, Tingting;Zhu, Yiyong;Routledge, Michael N.;Yang, Cuifeng
关键词：Agrochemical; Heavy metal; Metagenomics; Risk assessment; Soil fertility; Sustainable agriculture
-
Fluorescent and Colorimetric Dual-Readout Immunochromatographic Assay for the Detection of Phenamacril Residues in Agricultural Products
作者：Liu, Pengyan;Jiang, Lan;Wang, Yulong;Ye, Yuhui;Zhang, Cunzheng;Liu, Pengyan;Jiang, Lan;Wang, Yulong;Ye, Yuhui;Zhang, Cunzheng;Liu, Pengyan;Jiang, Lan;Wang, Yulong;Ye, Yuhui;Zhang, Cunzheng;Jiang, Lan;Zhang, Cunzheng;Zhao, Yun;Xue, Feng;Hammock, Bruce D.;Hammock, Bruce D.
关键词：fluorescent and colorimetric dual-readout; immunochromatographicassay; phenamacril; monoclonal antibody; polydopamine coated gold nanoparticles; quantum dots
-
Preparation of monoclonal antibody and establishment of indirect competitive chemiluminescence enzyme immunoassay for sodium pentachlorophenolate
作者：Dong, Sa;Shi, Qiuyun;Guan, Lingjun;Feng, Jianguo;Guan, Lingjun;Wang, Yulong;Liu, Pengyan;Zhang, Cunzheng
关键词：Sodium pentachlorophenate; Monoclonal antibody; ic-CLEIA; Residual detection; Animal tissues
-
Colorimetric and Reverse Fluorescence Dual-Signal Readout Immunochromatographic Assay for the Sensitive Determination of Sibutramine
作者：Gui, Yun;Zhang, Cunzheng;Gui, Yun;Zhao, Yun;Liu, Pengyan;Wang, Yulong;Mao, Xinxin;Zhang, Cunzheng;Zhao, Yun;Peng, Chifang;Mao, Xinxin;Zhang, Cunzheng;Hammock, Bruce D.;Hammock, Bruce D.
关键词：
