Multisite Mutagenesis of 4-Hydroxyphenylpyruvate Dioxygenase (HPPD) Enhances Rice Resistance to HPPD Inhibitors and Its Carotenoid Contents
文献类型： 外文期刊
作者： Yang, Yuwen 1 ; Zhou, Zhenzhen 1 ; Liu, Tingli 3 ; Tan, Qing 4 ; Chen, Le 1 ; Wang, Jinyan 1 ; Qiu, Zeyu 1 ; Ling, Xitie 1 ; Chen, Tianzi 1 ; Yang, Xia 1 ; Liu, Qing 1 ; Guo, Dongshu 1 ; Zhang, Baolong 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Prov Key Lab Agrobiol, Zhongling St 50, Nanjing 210014, Peoples R China
2.Zhongshan Biol Breeding Lab, Nanjing 210014, Peoples R China
3.Nanjing Xiaozhuang Univ, Nanjing 211171, Peoples R China
4.Hainan Univ, Coll Ocean, Haikou 571101, Peoples R China
关键词： rice; herbicides; 4-hydroxyphenylpyruvate dioxygenase; resistance; rice blast; directed evolution
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.2； 五年影响因子：6.4 ）
ISSN： 0021-8561
年卷期： 2024 年 72 卷 40 期
页码：
收录情况： SCI
摘要： While frequently used herbicides display limited efficacy against herbicide-resistant weeds, it becomes imperative to explore novel herbicides that ensure both effective weed management and environmental safety. Though 4-hydroxyphenylpyruvate dioxygenase (HPPD) inhibitory herbicides like mesotrione are prevalent in maize weed management, their integration into rice production is hindered due to the inherent sensitivity of rice HPPD (OsHPPD). In this study, a mutant allele of OsHPPD featuring six amino acid substitutions, termed OsHPPD-6M, maintains enzymatic activity in 200 mu m mesotrione while the wild type can only withstand 1 mu m. Enzymatic assays in vitro indicated that the HPPD activity of OsHPPD-6M surpassed that of the WT by 2-fold through enhanced substrate-binding. Its overexpression in transgenic rice conferred greater tolerance to mesotrione, topramezone, and isoxaflutole by 36.7-, 41.6-, and 37.1-fold relative to that in the WT rice. Interestingly, these 6M-OE plants demonstrated substantially elevated contents of carotenoids compared to WT plants without a significant impact on agronomic traits.
- 相关文献
作者其他论文 更多>>
-
Trehalose-6-phosphate phosphatase expression and enzymatic properties of Fusarium graminearum
作者：Zhang, Xuebiao;Ni, Zhong;Chen, Le;Zhuang, Yiqing;Xu, Chao;Wu, Qinyan
关键词：Trehalose-6-phosphate; Trehalose-6-phosphate phosphatase; Heterologous expression; Fusarium graminearum
-
Paenibacillus mesotrionivorans sp. nov., a Mesotrione-Degrading Strain Isolated from Soil
作者：Song, Ye;Wu, Yan;Ruan, Luyao;Wan, Minglai;Liu, Bin;He, Jian;Chen, Leyao;Wan, Minglai;Zhang, Baolong;He, Jian;He, Jian
关键词：
-
Development of a Recombinase Polymerase Amplification Method Combined with a Lateral Flow Dipstick Assay for Rapid Detection of the Larch Pathogen Neofusicoccum laricinum
作者：Ju, Fangyi;Tan, Jiajin;Dai, Tingting;Qi, Zhongqiang;Liu, Tingli;Dai, Tingting
关键词：lateral flow dipstick;
Neofusicoccum laricinum ; rapid detection; recombinase polymerase amplification -
Identification of QTLs and a candidate gene affecting rice grain volume via high-density genetic mapping
作者：Sun, Zhiguang;Li, Jingfang;Li, Jian;Yang, Bo;Liu, Jinbo;Chen, Tingmu;Zhang, Yuqin;Lu, Baiguan;Liu, Yan;Wang, Baoxiang;Xu, Dayong;An, Hongzhou;Qiu, Zeyu
关键词：weedy rice; grain volume; QTL; high-density genetic map; ethylene receptor
-
Creating a Superior Wx Allele with Temperature-Responsive Amylose Regulation and a Novel Transcriptional Pattern in Rice via CRISPR/Cas9-Mediated Promoter Editing
作者：Yan, Jiali;Yu, Jiawen;Shen, Huimin;Chen, Zhuanzhuan;Fan, Xiaolei;Li, Qianfeng;Zhang, Changquan;Liu, Qing;Huang, Lichun;Liu, Qiaoquan;Yan, Jiali;Yu, Jiawen;Shen, Huimin;Chen, Zhuanzhuan;Fan, Xiaolei;Li, Qianfeng;Zhang, Changquan;Huang, Lichun;Liu, Qiaoquan;Zhou, Lihui;Chen, Zhuanzhuan;Fan, Xiaolei;Li, Qianfeng;Zhang, Changquan;Huang, Lichun;Liu, Qiaoquan
关键词：
Wx gene; amylose content; grain transparency; promoter editing; transcriptional pattern -
A five-year examination into the occurrence of herbicide-resistant barnyardgrass populations in paddy from Jiangsu Province, China
作者：Hu, Xuli;Xu, Yong;Mao, Honghao;Liu, Ziyue;Xiao, Yi;Li, Yongfeng;Yang, Xia;Hu, Xuli;Li, Chaofeng
关键词：
Echinochloa ; Herbicide; Resistance frequency; Multiple resistance -
Prime Editing of TaEPSPS Homeologs Enhances Wheat Resistance to Glyphosate
作者：Chen, Tianzi;Qiu, Zeyu;Zhang, Baolong;Chen, Tianzi;Qiu, Zeyu;Zhang, Baolong;Li, Yaning;Zou, Changsong
关键词：Wheat;
EPSPS gene; prime editing; glyphosate resistance
