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Evaluation of unprotected and rumen-protected L-carnitine in vitro and in vivo

文献类型: 外文期刊

作者: Zheng, Lin 1 ; Han, Rui 1 ; Jiang, Wei 3 ; Chen, Long 2 ; Yu, Wei 2 ; Zhong, Wei-guang 2 ; Wei, Bing-dong 2 ;

作者机构: 1.Jilin Agr Univ, Minist Educ, Key Lab Anim Prod Product Qual & Secur, Changchun, Jilin, Peoples R China

2.Jilin Acad Agr Sci, Inst Anim Nutr & Feed Sci, Gongzhuling, Jilin, Peoples R China

3.Yanbian Univ, Agr Coll, Yanji, Jilin, Peoples R China

关键词: L-carnitine; rumen protection; sheep; urea nitrogen; antioxidation

期刊名称:CANADIAN JOURNAL OF ANIMAL SCIENCE ( 影响因子:1.077; 五年影响因子:1.281 )

ISSN: 0008-3984

年卷期: 2022 年 102 卷 2 期

页码:

收录情况: SCI

摘要: This study aimed to evaluate the effectiveness of unprotected and rumen-protected L-carnitine through in vitro tests, rumen degradation tests, and in vivo tests. Twelve rumen-fistulated crossbred rams with similar body weights of 55 ?? 3.6 kg and ages of 3 ?? 0.2 yr old were divided into three treatment groups in a 3 ?? 3 Latin square design, G1 (basal diet with no additives), G2 (unprotected L-carnitine), or G3 (rumen-protected L-carnitine). Ruminal fluid and blood samples were collected before morning feeding on the last day of each experimental period (21 d). The percentage of L-carnitine remaining in the simulated rumen and abomasum and rumen increased with the increase in the wall material ratio (P < 0.05). L-carnitine supplementation decreased the plasma urea nitrogen concentration in the sheep (P < 0.05). G3 resulted in higher glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activities as well as higher total antioxidant capacity (T-AOC) and lower malondialdehyde (MDA) concentrations in plasma than G1, and the difference was significant among the groups (P < 0.01). Thus, L-carnitine in the rumen could be protected by encapsulation for a certain time. Unprotected and rumenprotected L-carnitine supplementation effectively enhanced the antioxidant capacity of sheep, and the antioxidant capacity of sheep supplemented with rumen-protected L-carnitine was higher than that of sheep supplemented with unprotected L-carnitine. R??sum?? : Cette etude avait pour but d???evaluer l???efficacite de protection de la L-carnitine dans le rumen au moyen de tests in vitro, de tests de degradation dans le rumen, et de tests in vivo. Douze beliers croises fistules dans le rumen ayant des poids corporels (55 ?? 3,6 kg) et des ??ges (3 ?? 0,2 an) semblables ont ete divises en trois groupes traitements dans un design experimental ?? carre latin 3 ?? 3 : G1 (di??te de base sans supplements), G2 (L-carnitine non protegee) ou G3 (L-carnitine avec protection dans le rumen). Les echantillons de liquide du rumen et de sang Le pourcentage de L-carnitine restant dans le rumen simule, l???abomasum et le rumen a augmente avec l???augmenplasmatique d???azote ureique des moutons (P < 0,05). Le groupe G3 ont montre des activites plus elevees de glutathione peroxydase (GSH-Px), de superoxyde dismutase (SOD), et de capacite totale anti-oxydante (T-AOC ??? ?? total antioxidant capacity ??) et de faibles concentrations de malondialdehyde (MDA) dans le plasma que le groupe G1, et la difference etait significative entre les groupes (P < 0,01). Donc, la L-carnitine dans le rumen peut ??tre protection dans le rumen ont effectivement ameliore la capacite anti-oxydante des moutons, et la capacite antioxydante des moutons ayant re??u les supplements de L-carnitine avec protection dans le rumen etait plus elevee

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