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Effect of magnesium ammonium phosphate on the expression of adhesion molecules in sheep renal tubular epithelial cells

文献类型： 外文期刊

作者： Liu, Ruo-Nan 1 ; Zou, Dong-Min 2 ; Tian, Meng-Yue 1 ; Li, Ke 1 ; Du, Jin-Liang 1 ; Liu, Mao-Jun 4 ; Ma, Yu-Zhong 1 ;

作者机构： 1.Hebei Agr Univ, Coll Vet Med, 2596 Lekai South St, Baoding 071000, Hebei, Peoples R China

2.Shanxi Agr Univ, Coll Vet Med, Taigu 030801, Shanxi, Peoples R China

3.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Int Joint Res Lab Fish Immunopharmacol, Wuxi 214081, Jiangsu, Peoples R China

4.Jiangsu Acad Agr Sci, Inst Vet Med, Nanjing 210014, Jiangsu, Peoples R China

关键词： Intercellular adhesion molecule-1; Magnesium ammonium phosphate; Osteopontin; Sheep renal tubular epithelial cell; Vascular cell adhesion molecule-1

期刊名称：RESEARCH IN VETERINARY SCIENCE （ 影响因子：2.534； 五年影响因子：2.382 ）

ISSN： 0034-5288

年卷期： 2021 年 138 卷

页码：

收录情况： SCI

摘要： Adhesion molecules play an important role in urinary calculus formation. The expressions of adhesion molecules in renal tubular has been reported in some animals. However, the role of adhesion molecules in the process of sheep urinary calculus formation is still unclear. The magnesium ammonium phosphate (MAP) is the main component of sheep urinary calculus. In this paper, the sheep renal tubular epithelial cells (RTECs) were isolated and treated with MAP, the expressions of osteopontin (OPN), intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and apoptosis-related indicators caspase-3, Bcl-2 and Bax in RTECs were observed, the viability of RTECs was detected by Cell Counting Kit-8 (CCK-8). The levels of superoxide dismutase (SOD) and malondialdehyde (MDA), and the expressions of inflammatory factors Interleukin-6 (IL-6), Interleukin-1 (IL-1), Interleukin-17 (IL-17) and tumor necrosis factor-alpha (TNF-alpha) were measured by enzyme-linked immunosorbent (ELISA). The histopathological observation of kidney in urolithiasis sheep was made. The results showed that MAP could reduce the viability and SOD activity, enhance the activity of MDA significantly and promote the expressions of IL-1, IL-6, IL-17 and TNF-alpha of RTECs. By western blot and qPCR methods, the expressions of ICAM-1, VCAM-1 and OPN increased in 48 h. In addition, the expression of caspase-3 increased significantly and the ratio of Bcl-2/Bax reduced with exposure to MAP. The renal tissue structure was seriously damaged, the RTECs in urolithiasis sheep were degenerative and necrotic.

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