Purification of Classical Swine Fever Virus E2 Subunit Vaccines Based on High Affinity Peptide Ligand
文献类型： 外文期刊
作者： Wang, Fangyu 1 ; Yu, Qiuying 2 ; Hu, Man 1 ; Xing, Guangxu 1 ; Zhao, Dong 1 ; Zhang, Gaiping 1 ;
作者机构： 1.Henan Acad Agr Sci, Henan Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China
2.Henan Agr Univ, Coll Food Sci & Technol, Zhengzhou 450002, Peoples R China
3.Henan Agr Univ, Coll Anim Sci & Vet Med, Zhengzhou 450002, Peoples R China
关键词： Classical swine fever virus; glycoprotein E2; computational design; affinity peptide ligand; purification; pro-tein-peptide screening
期刊名称：PROTEIN AND PEPTIDE LETTERS （ 影响因子：1.89； 五年影响因子：1.528 ）
ISSN： 0929-8665
年卷期： 2021 年 28 卷 5 期
页码：
收录情况： SCI
摘要： Background: The purification of expressed proteins is the most critical part of subunit -vaccine production. Protein-purification methods such as affinity chromatography and exchange still have the shortcomings of being time consuming and complicated. With the rapid development of computational molecular-simulation technology, structure-based peptide-ligand design has become feasible. Objection: We aimed to apply molecular docking for a peptide ligand designed for classical swine fever virus (CSFV) E2 purification. Methods: Computational-derived peptides were synthesized, and the in vitro binding interaction with E2 was investigated. The effects of purification on E2 were also evaluated. Results: The best peptide recognizing E2 was P-6, which had a sequence of KKFYWRYWEH. Based on kinetic surface plasmon resonance (SPR) analysis, the apparent affinity constant of P-6 was found to be 148 nM. Importantly, P-6 showed suitable binding affinity and specificity for E2 purification from transgenic rice seeds. Evaluation of immune antibodies in mice showed that the antibody-blocking rate on day 42 after inoculation reached 86.18% and 90.68%. Conclusion: The computational-designed peptide in this study has high sensitivity and selectivity and is thus useful for the purification of CSFV E2. The novel method of design provided a broad platform and powerful tool for protein-peptide screening, as well as new insights into CSFV vaccine design.
- 相关文献
作者其他论文 更多>>
-
Enhancing humoral and mucosal immune response of PED vaccine candidate by fusing S1 protein to nanoparticle multimerization
作者：Li, Minghui;Sun, Xueke;Wang, Siqiao;Wang, Yanan;Wang, Yue;Zhang, Gaiping;Li, Minghui;Sun, Xueke;Chen, Yilan;Wang, Siqiao;Li, Qin;Wang, Yanan;Wang, Yue;Li, Ruiqi;Zhang, Gaiping;Ding, Peiyang;Zhang, Gaiping;Ding, Peiyang;Zhang, Gaiping;Zhang, Gaiping;Zhang, Gaiping
关键词：PEDV; S1 protein; Nanoparticle; Subunit vaccine; Mucosal immunity
-
Pseudorabies virus tegument protein US2 antagonizes antiviral innate immunity by targeting cGAS-STING signaling pathway
作者：Kong, Zhengjie;Zhang, Yifeng;Guan, Kaifeng;Yao, Wanzi;Kang, Yu;Lu, Xinyi;Zhang, Gaiping;Chen, Xing;Gong, Lele;Wang, Lele;Zhang, Yuhang;Du, Yongkun;Sun, Aijun;Zhuang, Guoqing;Wan, Bo;Zhang, Gaiping;Zhao, Jianguo;Zhang, Gaiping;Zhang, Gaiping
关键词：pseudorabies virus; cGAS-STING; tegument protein US2; TRIM21; immune invasion
-
IFI16 Positively Regulates RIG-I-Mediated Type I Interferon Production in a STING-Independent Manner
作者：Shi, Xibao;Wei, Menglu;Feng, Yuwen;Yang, Yuanhao;Zhang, Xiaozhuan;Chen, Hao;Xing, Yuqi;Wang, Keqi;Wang, Wensheng;Wang, Li;Zhang, Gaiping;Wang, Aiping;Zhang, Gaiping;Zhang, Gaiping;Zhang, Gaiping;Shi, Xibao;Zhang, Gaiping
关键词：IFN-gamma-inducible protein 16; interferon gene-stimulating protein; retinoic acid-inducible gene I; type I interferon
-
In Vitro Protective Effect of Pea-Derived Peptides (PPs) via the Keap1/Nrf2 Signaling Pathway on Alpha-Gliadin-Sensitizing Peptide Induced Cacao-2 Cells
作者：Gao, Bing;Yan, Fang;Wang, Chunfeng;Cui, Chenxu;Sun, Xuefeng;Wang, Fangyu;Li, Ning
关键词：antioxidant enzymes; celiac disease; free radicals scavenging; alpha-gliadin peptide; Keap1/Nrf2 pathway
-
A universal design of restructured dimer antigens: Development of a superior vaccine against the paramyxovirus in transgenic rice
作者：Ma, Fanshu;Zhang, Longxian;Zhang, Erqin;Zhang, Gaiping;Ma, Fanshu;Zhang, Gaiping;Ma, Fanshu;Zhang, Gaiping;Ma, Fanshu;Zhang, Gaiping;Ma, Fanshu;Xu, Qianru;Yang, Daichang;Li, Qingmei;Guo, Junqing;Li, Rui;Wang, Li;Wang, Yanan;Zhao, Xiangyue;Chai, Shujun;Yin, Heng;Li, Kunpeng;Rao, Zihe;Rao, Zihe;Rao, Zihe
关键词：structural vaccine; transgenic rice; BCR; paramyxovirus
-
Double-layered N-S1 protein nanoparticle immunization elicits robust cellular immune and broad antibody responses against SARS-CoV-2
作者：Li, Ruiqi;Chang, Zejie;Cheng, Anchun;Zhang, Gaiping;Li, Ruiqi;Zhang, Gaiping;Li, Ruiqi;Zhang, Gaiping;Li, Ruiqi;Chang, Zejie;Wang, Yanan;Li, Minghui;Chen, Yilan;Fan, Lu;Wang, Siqiao;Sun, Xueke;Liu, Siyuan;Zhang, Gaiping;Chang, Zejie;Wang, Yanan;Li, Minghui;Sun, Xueke;Liu, Siyuan;Zhang, Gaiping;Liu, Hongliang;Ding, Peiyang;Zhang, Gaiping
关键词：COVID-19; Coronavirus; SARS-CoV-2; Nanoparticle; Subunit vaccine; Variants
-
Porcine reproductive and respiratory syndrome virus triggers Golgi apparatus fragmentation-mediated autophagy to facilitate viral self-replication
作者：Zhao, Shuang-shuang;Qian, Qisheng;Zhang, Gaiping;Zhao, Shuang-shuang;Qian, Qisheng;Chen, Xin-xin;Lu, Qingxia;Xing, Guangxu;Qiao, Songlin;Li, Rui;Zhang, Gaiping;Zhang, Gaiping
关键词：autophagy; GA fragmentation; GRASP65; Nsp2; PRRSV; RAB2; ULK1
