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Evaluation of a DNA vaccine candidate co-expressing GP3 and GP5 of porcine reproductive and respiratory syndrome virus (PRRSV) with interferon alpha/gamma in immediate and long-lasting protection against HP-PRRSV challenge

文献类型： 外文期刊

作者： Du, Yijun 1 ; Qi, Jing 1 ; Lu, Yu; Wu, Jiaqiang 1 ; Yoo, Dongwan; Liu, Xing 1 ; Zhang, Xiumei 1 ; Li, Jun 1 ; Sun, Wen 1 ;

作者机构： 1.Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, Shandong Key Lab Anim Dis Control & Breeding, Jinan 250100, Peoples R China

2.Shandong Acad Agr Sci, I

关键词： PRRSV;GP3;GP5;Interferon alpha/gamma;Immune responses

期刊名称：VIRUS GENES （ 影响因子：2.332； 五年影响因子：2.0 ）

ISSN： 0920-8569

年卷期： 2012 年 45 卷 3 期

页码：

收录情况： SCI

摘要： Porcine reproductive and respiratory syndrome virus (PRRSV) has become one of the most economically important diseases to the global pork industry. Current vaccination strategies only provide a limited protective efficacy. In this study, a DNA vaccine, pVAX1(A (c))-alpha-gamma-GP35, co-expressing GP3 and GP5 of PRRSV with interferon alpha/gamma was constructed, and its immediate and long-lasting protection against highly pathogenic PRRSV (HP-PRRSV) challenge were examined in pigs. For immediate protection, the results showed that pVAX1(A (c))-alpha-gamma-GP35 could provide partially protective efficacy, which was similar to the pVAX1(A (c))-alpha-gamma (expressing interferon alpha/gamma). For long-lasting protection, pigs inoculated with pVAX1(A (c))-alpha-gamma-GP35 developed significantly higher PRRSV-specific antibody response, T cell proliferation, IFN-gamma, and IL-4, than those vaccinated with pVAX1(A (c))-GP35 (expressing GP3 and GP5 of PRRSV). Following homologous challenge with HP-PRRSV strain SD-JN, pigs inoculated with pVAX1(A (c))-alpha-gamma-GP35 showed almost no clinical signs, no lung lesions, and significantly lower viremia, as compared to those in pVAX1(A (c))-GP35 group. It indicated that pVAX1(A (c))-alpha-gamma-GP35 could induce enhanced immune responses and provide both immediate and long-lasting protection against HP-PRRSV challenge in pigs. The DNA vaccine pVAX1(A (c))-alpha-gamma-GP35 might be an attractive candidate vaccine for the prevention and control of HP-PRRSV infections.

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