您好，欢迎访问广东省农业科学院 机构知识库！
广东省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

LuxS/AI-2 in Streptococcus agalactiae reveals a key role in acid tolerance and virulence

文献类型： 外文期刊

作者： Ma, Yanping 1 ; Hao, Le 1 ; Ke, Hao 1 ; Liang, Zhiling 1 ; Ma, Jiangyao 1 ; Liu, Zhenxing 1 ; Li, Yugu 2 ;

作者机构： 1.Guangdong Acad Agr Sci, Inst Anim Hlth, Guangdong Prov Key Lab Livestock Dis Prevent, Guangdong Open Lab Vet Publ Hlth, Guangzhou 510640, Guangdong, Peoples R China

2.South China Agr Univ, Coll V

关键词： Tilapia Streptococcus agalactiae;LuxS;AI-2;Acid tolerance;Virulence

期刊名称：RESEARCH IN VETERINARY SCIENCE （ 影响因子：2.534； 五年影响因子：2.382 ）

ISSN： 0034-5288

年卷期： 2017 年 115 卷

页码：

收录情况： SCI

摘要： LuxS-mediated autoinducer-2 (AI-2) directly or indirectly regulates important physiologic function in a variety of bacteria. We found a luxS homologue in the genome of Streptococcus agalactiae, an important pathogen of tilapia. To investigate the relationship between luxS/AI-2 and pathogenicity for tilapia, its bioluminescent activity, acid resistance, cell adherence, virulence, and regulation of virulence gene were evaluated. Compared with the wild-type strain, the bioluminescent activity lost in the luxS mutant, its resistance to acid (pH 2.8) was significantly decreased 33.8 times, and furthermore, its adherence to the NGF-2 cell line was dramatically reduced 3 times in the mutant strain. The virulence of the mutant strain was decreased in the tilapia infection model, exogenous AI-2 molecule (7.4 nM) and luxS gene complementation with plasmid could complement the deficiencies of function in the luxS mutant strain. These results showed that inactivation of luxS gene caused a significant decrease of bioluminance, acid resistance, cell adhesion, virulence to tilapia and transcription levels of many virulence genes in S. agalactiae. Expression of the known stress resistance factors DnaK and GroEL, relative regulator factors CovR/CovS and virulence factor cpsE verified above results. These findings suggest that luxS may be involved in the interruption of bacterial virulence and resistance to environmental factors.

  • 相关文献

[1]luxS/AI-2 Quorum Sensing Is Involved in Antimicrobial Susceptibility in Streptococcus agalactiae. Ma, Yan Ping,Ke, Hao,Hao, Le,Liu, Zhen Xing,Liang, Zhi Ling,Ma, Jiang Yao,Ma, Yan Ping,Ke, Hao,Hao, Le,Liu, Zhen Xing,Liang, Zhi Ling,Ma, Jiang Yao,Yang, Hu Cheng,Li, Yu Gu.

[2]Protective efficacy of cationic-PLGA microspheres loaded with DNA vaccine encoding the sip gene of Streptococcus agalactiae in tilapia. Ma, Yan-ping,Ke, Hao,Liang, Zhi-ling,Ma, Jiang-yao,Hao, Le,Liu, Zhen-xing.

[3]Genome and Molecular Characterization of a CSFV Strain Isolated from a CSF Outbreak in South China. Shen, Hai-Yan,Wang, Jia-Ying,Dong, Xiao-Ying,Zhao, Ming-Qiu,Kang, Yanmei,Li, Yin-Guang,Pei, Jing-Jing,Liao, Ming,Ju, Chun-Mei,Yi, Lin,Hu, Yongming,Chen, Jin-Ding,Shen, Hai-Yan. 2013

[4]Functional characterization of the gene FoOCH1 encoding a putative alpha-1,6-mannosyltransferase in Fusarium oxysporum f. sp cubense. Li, Min-Hui,Xie, Xiao-Ling,Lin, Xian-Feng,Shi, Jin-Xiu,Ding, Zhao-Jian,Ling, Jin-Feng,Xi, Ping-Gen,Zhou, Jia-Nuan,Jiang, Zi-De,Leng, Yueqiang,Zhong, Shaobin,Ling, Jin-Feng. 2014

[5]FocVel1 influences asexual production, filamentous growth, biofilm formation, and virulence in Fusarium oxysporum f. sp cucumerinum. Li, Peidian,Pu, Xiaoming,Yang, Qiyun,Shen, Huifang,Zhang, Jingxin,Lin, Birun,Li, Peidian,Feng, Baozhen,Li, Peidian. 2015

[6]Genomic Analysis of Phylotype I Strain EP1 Reveals Substantial Divergence from Other Strains in the Ralstonia solanacearum Species Complex. Li, Peng,Wang, Dechen,Yan, Jinli,Zhou, Jianuan,Deng, Yinyue,Jiang, Zide,Zhang, Lianhui,Deng, Yinyue,Cao, Bihao,He, Zifu,Zhang, Lianhui. 2016

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广东省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台