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A Rapid and Cost Effective Method for Genotyping Two Types of GHR Mutations in Sex-Linked Dwarf Chicken

文献类型： 外文期刊

作者： Wang, Yan 2 ; Qu, Hao 2 ; Luo, Cheng-Long 2 ; Wang, Jie 2 ; Shu, Ding-Ming 1 ;

作者机构： 1.Guangdong Acad Agr Sci, Inst Anim Sci, Anim Breeding & Genet Grp, Guangzhou 510640, Guangdong, Peoples R China

2.State Key Lab Livestock & Poultry Breeding, Guangzhou 510640, Guangdong, Peoples R China

关键词： GHR;sex-linked dwarf chicken;genotyping;mutation;stable

期刊名称：JOURNAL OF ANIMAL AND VETERINARY ADVANCES （ 影响因子：0.365； 五年影响因子：0.359 ）

ISSN： 1680-5593

年卷期： 2012 年 11 卷 11 期

页码：

收录情况： SCI

摘要： Large deletion or mutation at the exon 10 and 3' untranslated region of Growth Factor Receptor (GHR) gene results in dwarf chicken and the common method for detecting mutation is direct PCR amplification. But since, the target fragment is large, the amplification is inefficient and unreliable and this process consumes a lot of expensive specific DNA polymerases. Researchers focused on the point mutation C146280A in the deletion fragment and set up a new method to detect large deletion and point mutation in this region. Comparing with direct PCR this new method which can detect both large deletion and point mutation with regular low-cost DNA polymerases is more stable, reliably and cheapness.

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