GRAS family member LATERAL SUPPRESSOR regulates the initiation and morphogenesis of watermelon lateral organs
文献类型： 外文期刊
作者： Jiang, Yanxin 1 ; Zhang, Anran 1 ; He, Wenjing 1 ; Li, Qingqing 1 ; Zhao, Bosi 1 ; Zhao, Hongjiao 1 ; Ke, Xubo 1 ; Guo, Yalu 1 ; Sun, Piaoyun 1 ; Yang, Tongwen 1 ; Wang, Zheng 1 ; Jiang, Biao 1 ; Shen, Junjun 1 ; Li, Zheng 1 ;
作者机构： 1.Northwest A&F Univ, Coll Hort, Yangling 712100, Shaanxi, Peoples R China
2.Guangdong Acad Agr Sci, Vegetable Res Inst, Guangzhou 510640, Peoples R China
期刊名称：PLANT PHYSIOLOGY （ 影响因子：7.4； 五年影响因子：8.7 ）
ISSN： 0032-0889
年卷期： 2023 年
页码：
收录情况： SCI
摘要： The lateral organs of watermelon (Citrullus lanatus), including lobed leaves, branches, flowers, and tendrils, together determine plant architecture and yield. However, the genetic controls underlying lateral organ initiation and morphogenesis remain unclear. Here, we found that knocking out the homologous gene of shoot branching regulator LATERAL SUPPRESSOR in watermelon (ClLs) repressed the initiation of branches, flowers, and tendrils and led to developing round leaves, indicating that ClLs undergoes functional expansion compared with its homologs in Arabidopsis (Arabidopsis thaliana), rice (Oryza sativa), and tomato (Solanum lycopersicum). Using ClLs as the bait to screen against the cDNA library of watermelon, we identified several ClLs-interacting candidate proteins, including TENDRIL (ClTEN), PINOID (ClPID), and APETALA1 (ClAP1). Protein-protein interaction assays further demonstrated that ClLs could directly interact with ClTEN, ClPID, and ClAP1. The mRNA in situ hybridization assay revealed that the transcriptional patterns of ClLs overlapped with those of ClTEN, ClPID, and ClAP1 in the axillary meristems and leaf primordia. Mutants of ClTEN, ClPID, and ClAP1 generated by the CRISPR/Cas9 gene editing system lacked tendrils, developed round leaves, and displayed floral diapause, respectively, and all these phenotypes could be observed in ClLs knockout lines. Our findings indicate that ClLs acts as lateral organ identity protein by forming complexes with ClTEN, ClPID, and ClAP1, providing several gene targets for transforming the architecture of watermelon. The GRAS family transcription factor LATERAL SUPPRESSOR, together with PINOID, TENDRIL, and APETALA1, controls axillary organ formation in watermelon.
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