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Disruption of the Myostatin Gene in Porcine Primary Fibroblasts and Embryos Using Zinc-Finger Nucleases

文献类型： 外文期刊

作者： Huang, Xian-Ju 1 ; Zhang, Hong-Xiao 1 ; Wang, Huili 2 ; Xiong, Kai 1 ; Qin, Ling 1 ; Liu, Honglin 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Anim Sci & Technol, Nanjing, Jiangsu, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Anim Sci, Nanjing, Jiangsu, Peoples R China

关键词： gene;knockout;myostatin;porcine;zinc-finger nucleases (ZFNs)

期刊名称：MOLECULES AND CELLS （ 影响因子：5.034； 五年影响因子：4.932 ）

ISSN： 1016-8478

年卷期： 2014 年 37 卷 4 期

页码：

收录情况： SCI

摘要： Myostatin represses muscle growth by negatively regulating the number and size of muscle fibers. Myostatin loss-of-function can result in the double-muscling phenotype and increased muscle mass. Thus, knockout of myostatin gene could improve the quality of meat from mammals. In the present study, zinc finger nucleases, a useful tool for generating gene knockout animals, were designed to target exon 1 of the myostatin gene. The designed ZFNs were introduced into porcine primary fibroblasts and early implantation embryos via electroporation and microinjection, respectively. Mutations around the ZFNs target site were detected in both primary fibroblasts and blastocysts. The proportion of mutant fibroblast cells and blastocyst was 4.81% and 5.31%, respectively. Thus, ZFNs can be used to knockout myostatin in porcine primary fibroblasts and early implantation embryos.

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