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Development of an immunochromatographic lateral flow test strip for detection of beta-adrenergic agonist Clenbuterol residues

文献类型： 外文期刊

作者： Zhang, GP 1 ; Wang, XN 2 ; Yang, JF 2 ; Yang, YY 2 ; Xing, GX 2 ; Li, QM 2 ; Zhao, D 3 ; Chai, SJ; Guo, JQ;

作者机构： 1.Henan Inst Sci & Technol, Coll Vet & Anim Sci, Xinxiang 453003, Peoples R China

2.Henan Inst Sci & Technol, Coll Vet & Anim Sci, Xinxiang 453003, Peoples R China; Henan Acad Agr Sci, Henan Prov Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China

3.Henan Inst Sci & Technol, Coll Vet & Anim Sci, Xinxiang 453003, Peoples R China; Henan Acad Agr Sc

关键词： Clenbuterol;test strip;colloidal gold;immunochromatography;rapid test

期刊名称：JOURNAL OF IMMUNOLOGICAL METHODS （ 影响因子：2.303； 五年影响因子：2.556 ）

ISSN： 0022-1759

年卷期： 2006 年 312 卷 1-2 期

页码：

收录情况： SCI

摘要： A rapid immunochromatographic lateral flow test strip was developed in a competitive format with the gold-conjugated monoclonal antibody to specifically determine the residues of Clenbuterol (CL), a beta-adrenergic agonist. The test strip is made up of a sample pad, a conjugate reagent pad, a test membrane containing a control line and a test line, and an absorbent pad. CL standard samples of 0, 0.1, 0.3, 0.9, 2.7, 8.1 ng/ml in swine urine were determined by the test strip. It was shown that detection limit of the test strip was as low as 0.1 ng/ml of CL and that the half of maximal inhibition concentration (IC50) in relative optical density was calculated to be 1.78 +/- 0.17 ng/ml under an optical density scanner. The sensitivity by eye measurement was 1.0 ng/ml. It takes 10 min to accomplish a test. Parallel analysis of urine samples from pigs fed with CL showed comparable results obtained from the test strip and GC-MS. Therefore, the test strip is very useful as a screening method for quantitative, semi-quantitative or qualitative detection of CL residues in swine urine. (c) 2006 Elsevier B.V. All rights reserved.

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