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Molecular cloning and identification of full-length cDNA encoding high affinity Fc receptor for bovine IgG (Fc gamma RI)

文献类型: 外文期刊

作者: Yan, YH 1 ; Li, XW 1 ; Wang, AP 1 ; Zhang, GP 1 ;

作者机构: 1.Henan Acad Agr Sci, Inst Anim & Vet Sci, Zhengzhou 450002, Peoples R China

关键词: cloning;identification;cattle;Fc gamma RI

期刊名称:VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY ( 影响因子:2.046; 五年影响因子:2.217 )

ISSN: 0165-2427

年卷期: 2000 年 75 卷 1-2 期

页码:

收录情况: SCI

摘要: The receptor I for the Fc region of immunoglobulin G (Fc gamma RI) is a member of the Ig superfamily with a high affinity, and it mediates antibody-dependent cellular cytotoxicity and immune complex clearance. In this study, a cDNA encoding the bovine Fc gamma RI was cloned. The full-length cDNA sequence is 1050 bp long with a short 5'- and long 3'-untranslated end regions, which codes for 349 amino acids and contains a signal peptide, an extracellular region with three Ig-like domains, and transmembrane and intracytoplasmic domains. Five potential N-linked glycosylation sites are recognized in this sequence. Compared with the sequences of human and mouse Fc gamma RI, the homologies of nucleotide sequences are 80 and 69% and homologies of deduced amino acid sequences are 66 and 55%, respectively. It is shown that the sequences of the monomeric IgG binding domain in these three species of Fc gamma RI are highly conserved. (C) 2000 Elsevier Science B.V. All rights reserved.

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