Development and evaluation of an immunochromatographic strip for trichinellosis detection
文献类型： 外文期刊
作者： Zhang, GP 1 ; Guo, JQ 2 ; Wang, XN 2 ; Yang, JX 2 ; Yang, YY 2 ; Li, QM 2 ; Li, XW 3 ; Deng, RG; Xiao, ZJ; Yang, JF; Xing 1 ;
作者机构： 1.Henan Acad Agr Sci, Henan Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China
2.Henan Acad Agr Sci, Henan Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China; Henan Inst Sci & Technol, Coll Vet & Anim Sci, Xinxiang 453003, Peoples R China
3.Henan Acad Agr Sci, Henan Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China; Henan Inst Sci & Technol, Coll Vet & A
关键词： Trichinella;immunochromatographic strip;excretory-secretory antigens
期刊名称：VETERINARY PARASITOLOGY （ 影响因子：2.738； 五年影响因子：2.951 ）
ISSN： 0304-4017
年卷期： 2006 年 137 卷 3-4 期
页码：
收录情况： SCI
摘要： In the present study, an immunochromatographic strip was developed for the serological detection of trichinellosis in swine. In the strip, the excretory-secretory (ES) antigens of Trichinella labelled with colloidal gold was used as the detector, and the staphylococcal protein A (SPA) and goat anti-ES antibody were blotted on the nitrocellulose membrane for the test and control lines, respectively. The evaluation of the strip was performed by comparing 60 clinical positive blood samples detected by the artificial digestion method with 46 serum samples from pigs infected with parasites other than Trichinella and 30 serum samples of parasite-free healthy pigs. The strip was shown to be of high specificity and sensitivity that were closely correlated with those of ELISA. Furthermore, the dipstick assay based on the strip is rapid (10 min) and easy to perform with no requirement of special skill, reagent or equipment. This suggests the immunochromatographic strip is an acceptable alternative to be used in clinical laboratories lacking specialized equipment as well as for field diagnosis. (c) 2006 Elsevier B.V. All rights reserved.
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