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Development of a one-step strip test for the diagnosis of chicken infectious bursal disease

文献类型： 外文期刊

作者： Zhang, GP 1 ; Li, QM 2 ; Yan, YY 2 ; Guo, JQ 2 ; Li, XW 2 ; Deng, RG 2 ; Xiao, ZJ 2 ; Xing, GX 2 ; Yang, JF 3 ; Zhao, D; Cai, S 1 ;

作者机构： 1.Henan Acad Agr Sci, Inst Biotechnol, Zhengzhou 450002, Peoples R China

2.Henan Acad Agr Sci, Inst Biotechnol, Zhengzhou 450002, Peoples R China; Henan Agr Univ, Zhengzhou 450002, Peoples R China

3.Henan Acad Agr Sci, Inst Biotechnol, Zhengzhou 450002, Peoples R China; Henan Agr Univ, Zhe

关键词： infectious bursal disease;rapid diagnostic strip;colloidal gold

期刊名称：AVIAN DISEASES （ 影响因子：1.577； 五年影响因子：1.915 ）

ISSN： 0005-2086

年卷期： 2005 年 49 卷 2 期

页码：

收录情况： SCI

摘要： A rapid diagnostic strip for chicken infectious bursal disease (IBD) was developed based on membrane chromatography using high-affinity monoclonal antibodies directed to chicken infectious bursal disease virus (IBDV). The diagnostic strip has high specificity for detection of chicken IBDV antigen and recognizes a variety of the virus isolates, including virulent and attenuated strains, with no cross-reactivity to other viruses, such as Newcastle disease virus, Marek's disease virus, infectious bronchitis virus, infectious laryngotracheitis virus, and egg-drop-syndrome virus. The results showed that its specificity was highly consistent with the agar-gel precipitation test (AGP). The diagnostic strip detected as low as 800 median egg lethal dose (ELD50) viruses in the IBDV BC6/85-infected sample, which was comparable with AC-ELISA (400 ELD50) and 32 times more sensitive than the AGP test (2.56 x 10(4) ELD50). In experimental infection, IBDV was detected in the bursa as early as 36 hr postinfection with the diagnostic strip before the clinical signs and gross lesions appeared. It takes only 1-2 min to do a strip test to detect chicken IBDV antigen after the specimen is grounded in a whirl pack with finger massage.

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[1]High level soluble expression and one-step purification of IBDV VP2 protein in Escherichia coli. Jiang, Dawei,Zhang, Gaiping,Yang, Guoyu,Song, Yapeng,Wang, Guoqiang,Wang, Jucai,Zhao, Baolei,Jiang, Dawei,Liu, Yunchao,Zhang, Gaiping,Ji, Pengchao,Liu, Chang,Song, Yapeng,Su, Yunfang,Wang, Guoqiang,Wang, Jucai,Zhao, Baolei,Deng, Ruiguang,Wang, Aiping,Chen, Yumei.

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[3]Rapid and Sensitive Detection of the Food Allergen Glycinin in Powdered Milk Using a Lateral Flow Colloidal Gold Immunoassay Strip Test. Wang, Yao,Li, Zhixi,Deng, Ruiguang,Zhang, Gaiping,Li, Qingmei,Yang, Jifei,Sun, Yaning,Hu, Xiaofei,Zhang, Gaiping.

[4]Development of a Lateral Flow Colloidal Gold Immunoassay Strip for the Rapid Detection of Olaquindox Residues. Song, Chunmei,Liu, Qingtang,Zhi, Aimin,Yang, Jifei,Zhi, Yubao,Li, Qingmei,Hu, Xiaofei,Deng, Ruiguang,Zhang, Gaiping,Song, Chunmei,Casas, Justin,Tang, Liang.

[5]Establishment of a Lateral Flow Colloidal Gold Immunoassay Strip for the Rapid Detection of Soybean Allergen beta-Conglycinin. Wang, Yao,Li, Zhaozhou,Pei, Yafeng,Hou, Yuze,Wang, Yao,Li, Qingmei,Sun, Yaning,Yang, Jifei,Yang, Yanyan,Zhi, Yubao,Deng, Ruiguang,Hu, Xiaofei.

[6]Establishment of a lateral flow colloidal gold immunoassay strip for the rapid detection of estradiol in milk samples. Yang, Xing-dong,Wang, Fang-yu,Zhang, Gai-ping,Yang, Xing-dong,Zeng, Xian-yin,Song, Chun-mei,Wu, Shu-yan,Zhang, Gai-ping.

[7]Colloidal gold-McAb probe-based rapid immunoassay strip for simultaneous detection of fumonisins in maize. Yao, Jingjing,Sun, Yaning,Li, Qingmei,Wang, Fangyu,Teng, Man,Yang, Yanyan,Deng, Ruiguang,Hu, Xiaofei.

[8]Development of a colloidal gold-based strip test for the detection of chlorothalonil residues in cucumber. Yang, Xingdong,Zeng, Xianyin,Yang, Xingdong,Zhang, Gaiping,Wang, Fangyu,Wang, Yinbiao,Hu, Xiaofei,Li, Qingmei,Jia, Guochao,Liu, Zhen,Wang, Yao,Deng, Ruiguang,Zhang, Gaiping.

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[10]Development of an Immunochromatographic Strip Test for the Rapid Detection of Zearalenone in Corn. Sun, Yaning,Hu, Xiaofei,Yang, Jifei,Wang, Fangyu,Wang, Yao,Deng, Ruiguang,Zhang, Gaiping,Sun, Yaning,Zhang, Yong,Zhang, Gaiping.

[11]Development of an immunochromatographic assay for the rapid detection of chlorpyrifos-methyl in water samples. Hua, Xiude,Qian, Guoliang,Hu, Baishi,Fan, Jiaqin,Qin, Na,Li, Gang,Wang, Yuyan,Liu, Fengquan,Yang, Jifei.

[12]Development of an immunochromatographic lateral flow test strip for detection of beta-adrenergic agonist Clenbuterol residues. Zhang, GP,Wang, XN,Yang, JF,Yang, YY,Xing, GX,Li, QM,Zhao, D,Chai, SJ,Guo, JQ. 2006

[13]Development of a lateral-flow immunochromatographic test device for the rapid detection of difloxacin residues. Zhi, Ai-min,Li, Bin-bin,Liu, Qing-tang,Hu, Xiao-fei,Zhao, Dong,Hou, Yu-ze,Deng, Rui-guang,Zhang, Gai-ping,Li, Bin-bin,Chai, Shu-Jun. 2010

[14]Development of a lateral flow immunoassay strip for screening of sulfamonomethoxine residues. Zhang, G.,Wang, X.,Bao, Y.,Wang, Z.,Zhang, G.,Wang, X.,Yang, Y.,Luo, J.,Li, Q.,Guo, J.,Yang, J.,Xing, G.,Chai, S.,Shi, T.,Liu, Q.,Zhi, A.,Qu, M.. 2008

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