Determination of cordycepin content of Cordyceps militaris recombinant rice by high performance liquid chromatography
文献类型： 外文期刊
作者： Wang, Xuesong 1 ; Liu, Fang 1 ; Li, Fang 1 ; Cai, Hongyan 1 ; Sun, Wei 1 ; Chen, Xuan 1 ; Gao, Hong 3 ; Shen, Wangyang 1 ;
作者机构： 1.Wuhan Polytech Univ, Sch Food Sci & Technol, Wuhan, Peoples R China
2.Hubei Collaborat Innovat Ctr Proc Agr Prod, Wuhan 430023, Peoples R China
3.Hubei Acad Agr Sci, Inst Agriprod Proc & Nucl Agr Technol, Wuhan 430064, Peoples R China
关键词： Recombinant rice;Cordycepin;Chinese caterpillar fungus;Aweto;Cordyceps militaris
期刊名称：TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH （ 影响因子：0.533； 五年影响因子：0.743 ）
ISSN： 1596-5996
年卷期： 2016 年 15 卷 10 期
页码：
收录情况： SCI
摘要： Purpose: To assess the suitability of high performance liquid chromatography (HPLC) for the determination of cordycepin content of Cordyceps militaris recombinant rice Methods: Cordyceps militaris recombinant rice was made by mixing brown rice with artificial Chinese caterpillar fungus culture medium powder using twin-screw extrusion technology. Cordycepin content was determined by reversed-phase HPLC with water: acetonitrile (95: 5, v/v) as mobile phase, detection wavelength of 260 nm, and flow rate of 1.0 mL/min. Results: Cordycepin contents showed good linearity in the range of 1 - 50.0 mu g/mL (r(2) = 0.9996), and while recovery ranged from 103.2 to 109.9 %. Relative standard deviation (RSD), precision and repeatability RSD was 2.38, 0.76 and 1.46 %, respectively. Conclusion: The HPLC method is simple, fast, accurate and reproducible. It is suitable for determination of cordycepin content of artificial Chinese caterpillar fungus culture medium and brown rice recombinant rice.
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