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Candidate Resistant Genes of Sand Pear (Pyrus pyrifolia Nakai) to Alternaria alternata Revealed by Transcriptome Sequencing

文献类型： 外文期刊

作者： Yang, Xiaoping 1 ; Hu, Hongju 2 ; Yu, Dazhao 1 ; Sun, Zhonghai 2 ; He, Xiujuan 2 ; Zhang, Jingguo 2 ; Chen, Qiliang 2 ; T 1 ;

作者机构： 1.Wuhan Univ, Sch Life Sci, Wuhan 430072, Hubei, Peoples R China

2.Hubei Acad Agr Sci, Res Inst Fruit & Tea, Wuhan 430064, Hubei, Peoples R China

3.Hubei Lab Crop Dis Insect Pests & Weeds Control, Wuhan 430064, Hubei, Peoples R China

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2015 年 10 卷 8 期

页码：

收录情况： SCI

摘要： Pear black spot (PBS) disease, which is caused by Alternaria alternata (Aa), is one of the most serious diseases affecting sand pear (Pyrus pyrifolia Nakai) cultivation worldwide. To investigate the defense mechanisms of sand pear in response to Aa, the transcriptome of a sand pear germplasm with differential resistance to Aa was analyzed using Illumina paired-end sequencing. Four libraries derived from PBS-resistant and PBS-susceptible sand pear leaves were characterized through inoculation or mock-inoculation. In total, 20.5 Gbp of sequence data and 101,632,565 reads were generated, representing 44717 genes. Approximately 66% of the genes or sequenced reads could be aligned to the pear reference genome. A large number (5213) of differentially expressed genes related to PBS resistance were obtained; 34 microsatellites were detected in these genes, and 28 genes were found to be closely related to PBS resistance. Using a transcriptome analysis in response to PBS inoculation and comparison analysis to the PHI database, 4 genes (Pbr039001, Pbr001627, Pbr025080 and Pbr023112) were considered to be promising candidates for sand pear resistance to PBS. This study provides insight into changes in the transcriptome of sand pear in response to PBS infection, and the findings have improved our understanding of the resistance mechanism of sand pear to PBS and will facilitate future gene discovery and functional genome studies of sand pear.

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