您好，欢迎访问中国热带农业科学院 机构知识库！
中国热带农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Structure, Expression, and Functional Analysis of the Hexokinase Gene Family in Cassava

文献类型： 外文期刊

作者： Geng, Meng-Ting 1 ; Yao, Yuan 2 ; Wang, Yun-Lin 1 ; Wu, Xiao-Hui; Sun, Chong; Li, Rui-Mei 2 ; Fu, Shao-Ping 2 ; Dua 1 ;

作者机构： 1.Hainan Univ, Coll Agr, Haikou 570228, Peoples R China

2.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Minist Agr, Key Lab Biol & Genet Resources Trop Crops, Haikou 571101, Peoples R China

3.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Minist Agr, Key Lab Biol & Genet Resources Trop Crops, Haikou 571101, People

关键词： cassava;hexokinase;gene expression;yeast complementation;enzyme activities

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN： 1422-0067

年卷期： 2017 年 18 卷 5 期

页码：

收录情况： SCI

摘要： Hexokinase (HXK) proteins play important roles in catalyzing hexose phosphorylation and sugar sensing and signaling. To investigate the roles of HXKs in cassava tuber root development, seven HXK genes (MeHXK1-7) were isolated and analyzed. A phylogenetic analysis revealed that the MeHXK family can be divided into five subfamilies of plant HXKs. MeHXKs were clearly divided into type A (MeHXK1) and type B (MeHXK2-7) based on their N-terminal sequences. MeHXK1-5 all had typical conserved regions and similar protein structures to the HXKs of other plants; while MeHXK6-7 lacked some of the conserved regions. An expression analysis of the MeHXK genes in cassava organs or tissues demonstrated that MeHXK2 is the dominant HXK in all the examined tissues (leaves, stems, fruits, tuber phloems, and tuber xylems). Notably, the expression of MeHXK2 and the enzymatic activity of HXK were higher at the initial and expanding tuber stages, and lower at the mature tuber stage. Furthermore, the HXK activity of MeHXK2 was identified by functional complementation of the HXK-deficient yeast strain YSH7.4-3C (hxk1, hxk2, glk1). The gene expression and enzymatic activity of MeHXK2 suggest that it might be the main enzyme for hexose phosphorylation during cassava tuber root development, which is involved in sucrose metabolism to regulate the accumulation of starch.

  • 相关文献

[1]Identification, Expression, and Functional Analysis of the Fructokinase Gene Family in Cassava. Yao, Yuan,Li, Rui-Mei,Fu, Shao-Ping,Duan, Rui-Jun,Liu, Jiao,Guo, Jian-Chun,Geng, Meng-Ting,Wang, Yun-Lin,Chen, Xia,Shang, Lu,Lu, Xiao-Hua,Li, Zhan,Hu, Xin-Wen,Wu, Xiao-Hui,Sun, Chong. 2017

[2]Genome-Wide Identification, 3D Modeling, Expression and Enzymatic Activity Analysis of Cell Wall Invertase Gene Family from Cassava (Manihot esculenta Crantz). Yao, Yuan,Geng, Meng-Ting,Liu, Jiao,Li, Rui-Mei,Guo, Jian-Chun,Wu, Xiao-Hui,Hu, Xin-Wen. 2014

[3]Genome-Wide Identification, Expression, and Activity Analysis of Alkaline/Neutral Invertase Gene Family from Cassava (Manihot esculenta Crantz). Yao, Yuan,Geng, Meng-Ting,Liu, Jiao,Li, Rui-Mei,Guo, Jian-Chun,Yao, Yuan,Wu, Xiao-Hui,Hu, Xin-Wen.

[4]Genome-Wide Identification and Expression Analysis of the WRKY Gene Family in Cassava. Wei, Yunxie,Shi, Haitao,Xia, Zhiqiang,Tie, Weiwei,Ding, Zehong,Yan, Yan,Wang, Wenquan,Hu, Wei,Li, Kaimian. 2016

[5]Genome-wide gene phylogeny of CIPK family in cassava and expression analysis of partial drought-induced genes. Hu, Wei,Xia, Zhiqiang,Yan, Yan,Ding, Zehong,Tie, Weiwei,Zou, Meiling,Wei, Yunxie,Lu, Cheng,Hou, Xiaowan,Wang, Wenquan,Peng, Ming,Wang, Lianzhe. 2015

[6]Isolation and Characterization of Ftsz Genes in Cassava. Geng, Meng-Ting,Yao, Yuan,Li, Rui-Mei,Fu, Shao-Ping,Duan, Rui-Jun,Liu, Jiao,Guo, Jian-Chun,Geng, Meng-Ting,Min, Yi,Chen, Xia,Fan, Jie,Yuan, Shuai,Wang, Lei,Zhang, Fan,Shang, Lu,Wang, Yun-Lin,Hu, Xin-Wen,Sun, Chong. 2017

[7]Genome-Wide Identification and Expression Analysis of the KUP Family under Abiotic Stress in Cassava (Manihot esculenta Crantz). Ou, Wenjun,Li, Kaimian,Mao, Xiang,Huang, Chao,Tie, Weiwei,Yan, Yan,Ding, Zehong,Wu, Chunlai,Xia, Zhiqiang,Wang, Wenquan,Li, Kaimian,Hu, Wei,Zhou, Shiyi. 2018

[8]Isolation and characterization of a C-repeat binding factor (CBF)-like gene in cassava (Manihot esculenta Crantz). Li, Ruimei,Fan, Jie,Yang, Chenglong,Yao, Yuan,Zhou, Yang,Duan, Ruijun,Liu, Jiao,Fu, Shaoping,Guo, Jianchun,Li, Ruimei,Fan, Jie,Yang, Chenglong,Yao, Yuan,Zhou, Yang,Duan, Ruijun,Liu, Jiao,Fu, Shaoping,Guo, Jianchun,Ji, Yimeng,Fan, Jie,Yang, Chenglong,Yao, Yuan,Hu, Xinwen. 2014

[9]八个木薯品种(系)储藏根采后耐贮性生化指标的变化. 文明富,胡梅珍,陈新,王海燕,卢诚,王文泉. 2013

[10]Physiological Investigation and Transcriptome Analysis of Polyethylene Glycol (PEG)-Induced Dehydration Stress in Cassava. Fu, Lili,Ding, Zehong,Han, Bingying,Hu, Wei,Li, Yajun,Zhang, Jiaming. 2016

[11]Transgenic rice expressing a cassava (Manihot esculenta Crantz) plasma membrane gene MePMP3-2 exhibits enhanced tolerance to salt and drought stresses. Yu, Y.,Cui, Y. C.,Ren, C.,Rocha, P. S. C. F.,Wang, M. L.,Xia, X. J.,Yu, Y.,Ren, C.,Peng, M.,Xu, G. Y.. 2016

[12]Somatic Embryogenesis and Organogenesis of Biofuel Plant Cassava (Manihot esculenta Crantz) Chinese Cultivars. Li, Ruimei,Duan, Ruijun,Ji, Yimeng,Xi, Dujuan,Liu, Jiao,Guo, Jianchun,Fu, Shaoping,Li, Ruimei,Duan, Ruijun,Ji, Yimeng,Xi, Dujuan,Liu, Jiao,Guo, Jianchun,Fu, Shaoping,Ji, Yimeng,Xi, Dujuan,Liu, Jiao,Zhang, Peng. 2012

[13]Chilling acclimation provides immunity to stress by altering regulatory networks and inducing genes with protective functions in Cassava. Zeng, Changying,Chen, Xin,Zhou, Yufei,Bo, Weiping,Song, Shun,Deng, Deli,Guo, Xin,Wang, Bin,Wang, Wenquan,Peng, Ming,Chen, Zheng,Xia, Jing,Zhang, Kevin,Zhou, Junfei,Peng, Hai,Zhang, Weixiong,Chen, Zheng,Xia, Jing,Zhang, Kevin,Zhang, Weixiong,Zhang, Weixiong. 2014

[14]MeSAUR1, Encoded by a Small Auxin-Up RNA Gene, Acts as a Transcription Regulator to Positively Regulate ADP-Glucose Pyrophosphorylase Small Subunit1a Gene in Cassava. Ma, Ping'an,Liu, Chen,Meng, Yuhong,Ma, Ping'an,Chen, Xin,Liu, Chen,Meng, Yuhong,Xia, Zhiqiang,Zeng, Changying,Lu, Cheng,Wang, Wenquan,Ma, Ping'an,Chen, Xin,Liu, Chen,Meng, Yuhong,Xia, Zhiqiang,Zeng, Changying,Lu, Cheng,Wang, Wenquan. 2017

[15]Production of calcium gluconate from cassava by Penicillium citrinum SCG-112. Sun, Hai-Yan,Zhao, Pingjuan,Li, Juanhua,Liu, Enshi,Peng, Ming,Sun, Hai-Yan,Zhao, Pingjuan,Peng, Ming. 2011

[16]Quantitative Trait Locus Analysis for Yield Traits of Cassava (Manihot esculenta Crantz). Yan, Qingxiang,Li, Kaimian,Zhang, Xueqin,Ye, Jianqiu,Chen, Songbi,Li, Qing X.,Huang, Dongyi. 2014

[17]Cloning and Analysis of Vacuolar Invertase Gene (MeVINV2) Promoter from Cassava (Manihot Esculenta Crantz). Liu, Jiao,Hu, Yan-ping,Xia, Wen-rui,Yao, Yuan,Zhou, Yang,Fu, Shao-ping,Duan, Rui-jun,Li, Rui-mei,Guo, Jian-chun. 2014

[18]Change in physicochemical traits of cassava roots and starches associated with genotypes and environmental factors. Gu, Bi,Yao, Qingqun,Li, Kaimian,Chen, Songbi. 2013

[19]Validation of Reference Genes for Relative Quantitative Gene Expression Studies in Cassava (Manihot esculenta Crantz) by Using Quantitative Real-Time PCR. Hu, Meizhen,Wang, Wenquan,Hu, Meizhen,Xia, Zhiqiang,Zhou, Xincheng,Wang, Wenquan,Hu, Meizhen,Wang, Wenquan,Hu, Wenbin. 2016

[20]Environmental suitability of the red spider mite Tetranchus cinnabarinus (Acari: Tetranychidae) among cassava in China. Lu, Hui,Lu, Fuping,Xu, Xuelian,Chen, Qing. 2012

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国热带农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台