您好，欢迎访问中国热带农业科学院 机构知识库！
中国热带农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Toxicarioside A, isolated from tropical Antiaris toxicaria, blocks endoglin/TGF-beta signaling in a bone marrow stromal cell line

文献类型： 外文期刊

作者： Li, Yue-Nan 1 ; Huang, Feng-Ying 1 ; Mei, Wen-Li 1 ; Dai, Hao-Fu 1 ; Guo, Jun-Li 3 ; Tan, Guang-Hong 4 ; Zhou, Peng 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Peoples R China

2.Hainan Univ, Coll Agr, Haikou 570228, Peoples R China

3.Hainan Med Coll, Hainan Prov Key Lab Trop Med, Haikou 571101, Peoples R China

4.Hainan Med Coll, Hainan Prov Key Lab Trop Med, Haikou 571101,

关键词： Toxicarioside A;Endoglin;Transforming growth factor-beta;Signal pathway;Cell proliferation

期刊名称：ASIAN PACIFIC JOURNAL OF TROPICAL MEDICINE （ 影响因子：1.226； 五年影响因子：2.285 ）

ISSN： 1995-7645

年卷期： 2012 年 5 卷 2 期

页码：

收录情况： SCI

摘要： Objective: To investigate possible mechanism of toxicarioside A in HS-5 bone stromal cells. Methods: HS-5 bone stromal cells were cultured in media supplemented with various concentrations of toxicarioside A or control Dmso (not treatment). Endoglin and TGF were detected by Northern and Western blot analysis and quantified in a standard method. Downstream molecules of endoglin and TGF-beta (Smad1, Smad2 and their active phosphorlated counterparts, pSmad1 and pSmad2) were also detected and quantified by Western blot analysis. In addition, cell proliferation assay and small interfering RNA (siRNA) against endoglin were used to certificate the function of endolgin in the HS-5 cells. Results: Compared with the not treated (0 mu g/mL) or HMSO treated control HS-5 cells, HS-5 cells treated with toxicarioside A were found significant attenuation of endolgin and TGF p expression. Significant inhibition of cell proliferation was also found in the HS-5 cells treated with toxicarioside A. ALK1-related Smadl and ALK5-related Srnad2 were decreased in HS-5 cells treated with toxicarioside A. In addition, phosphorylated Smadl (pSmad1) and Smad2 (pSmad2) were also found attenuation in toxicarioside A treated HS-5 cells. RNA interference showed that blockage of endoglin by siRNA also decreased Smadl and Smad2 expression in HS-5 cells. Conclusions: Our results indicate that tosicatioside A can influence bone marrow stromal HS-5's function and inhibit HS-5 cell proliferation by alteration of endoglin-related ALK1 (Smadl) and ALIO (Smad2) signaling.

  • 相关文献

[1]Toxicarioside A inhibits SGC-7901 proliferation, migration and invasion via NF-kappa B/bFGF signaling. Mei, Wen-Li,Dai, Hao-Fu,Guo, Jun-Li,Zheng, Shao-Jiang,Li, Yue-Nan,Hao, Xin-Bao,Li, Tian-Fa,Xia, Li-Ping,Huang, Feng-Ying,Kong, Yue-Qiong,He, Qi-Yi,Yang, Kun,Tan, Guang-Hong,Jie, Wei. 2012

[2]Over-expression of mango (Mangifera indica L.) MiARF2 inhibits root and hypocotyl growth of Arabidopsis. Wu, Bei,Chen, Qi-Zhu,Huang, Xia,Chen, Yun-Feng,Huang, Xue-Lin,Li, Yun-He,Wu, Jian-Yong.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国热带农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台