Analysis of differentially expressed proteins in Muscovy duck embryo fibroblasts infected with virulent and attenuated Muscovy duck reovirus by two-dimensional polyacrylamide gel electrophoresis
文献类型： 外文期刊
作者： Huang, Mei-Qing 1 ; Cheng, Xiao-Xia 1 ; Chen, Shi-Long 1 ; Zheng, Min 1 ; Chen, Shao-Ying 1 ;
作者机构： 1.Fujian Acad Agr Sci, Anim Husb & Vet Med Inst, Fuzhou 350013, Fujian, Peoples R China
2.Fujian Anim Dis Control Technol Dev Ctr, Fuzhou 350013, Fujian, Peoples R China
关键词： 2D technology;muscovy duck embryo fibroblasts (MDEF);muscovy duck reovirus (MDRV);qRT-PCR
期刊名称：JOURNAL OF VETERINARY MEDICAL SCIENCE （ 影响因子：1.267； 五年影响因子：1.348 ）
ISSN： 0916-7250
年卷期： 2017 年 79 卷 12 期
页码：
收录情况： SCI
摘要： Muscovy duck reovirus (MDRV) belongs to the Orthoreovirus genus of the Reoviridae family, which is a significant poultry pathogen leading to high morbidity and mortality in ducklings. However, the pathogenesis of the virus is not well understood. In the present study, two-dimensional (2D) polyacrylamide gel electrophoresis (PAGE) combined with LC-MS-MS was used to identify differentially expressed proteins between Muscovy duck embryo fibroblasts (MDEF) infected with virulent (MV9710 strain) and attenuated (CA strain) MDRV and non-infected MDEFs. A total of 115 abundant protein spots were identified. Of these, 59 of differentially expressed proteins were detected, with functions in metabolism and utilization of carbohydrates and nucleotides, anti-stress, and regulation of immune and cellular process. GO analysis of the identified proteins showed that they belonged to the classes molecular function (141 proteins), cellular component (62 proteins), and biological process (146 proteins). The results were validated by qRT-PCR, which suggests that the analysis method of 2D PAGE combined with LC-MS-MS used in this study is reliable. This study lays a foundation for further investigation of the biology of MDRV infection in MDEF.
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