Expression in Insect Cells and Antigenic Analysis of Recombinant Nucleocapsid Protein of Canine Distemper Virus
文献类型： 会议论文
第一作者： Bi Zhenwei
作者： Bi Zhenwei 1 ; Wang Yongshan 1 ; Ouyang Wei 1 ; Fan HongJie 2 ;
作者机构： 1.Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences/Key Laboratory of Veterinary Biological Engineering and Technology,Ministry of Agriculture/National Center for Engineering Research of Veterinary Bio-products,Nanjing 210014,China
2.College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China
会议名称： International congress on animal hygiene
主办单位：
页码： 543-543
摘要： The nucleocapsid (N)protein gene of an atypical Canine distemper virus (CDV)was cloned into pFastBacHTA donor plasmid.The recombinant donor plasmid pFastBac-N containing N gene was constructed and transformed into competent E.coli DHIOBac cells that were grown on LB plate containing 3 antibiotics.Recombinant Bacmid DNA (rBacmid-N)was obtained and used to transfect insect cells Sf9 with Lipofectamine to produce baculovirus vBacmid-N.The expressed recombinant N protein band of approximate 62 kDa was detected in Western blot.The recombinant vBacmid-N antigen was visualized in infected Sf9 cells in indirect immunofluorescence assay.Purified recombinant N protein was used to establish indirect ELISA for the detection of antibody against CDV.The absorbance values at 450 nm of all CD-positive serum samples were above 0.4 whereas CD-negative serum samples were below 0.05.
分类号： s851.2
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