文献类型： 会议论文

第一作者： Bi Zhenwei

作者： Bi Zhenwei ¹ ; Wang Yongshan ¹ ; Xia Xingxia ¹ ; Sun Jing ¹ ; Fan Hongjie ² ;

作者机构： 1.Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences/Key Laboratory of Veterinary Biological Engineering and Technology,Ministry of Agriculture/National Center for Engineering Research of Veterinary Bio-products,Nanjing 210014,China

2.College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China

会议名称： International congress on animal hygiene

主办单位：

页码： 544-544

摘要： The splenocyte of BALB/c mice immunized with purified canine distemper virus (CDV)were fused with SP2/0 cells.Twelve hybridomas secreting monoclonal antibodies (mAbs)against CDV,designated as 3H11,ID7,2C9,1F8,G12,F7,H4,A2,2G3,2D9,3G12 and 3G3,were selected with inderect ELISA and cloned by the method of limiting dilution.Monoclonal antibodies secreted from hybridomas 3HII,ID7,2C9,IF8,G12,F7,H4,A2,2G3,2D9,3G12 and 3G3 were identified to be subclass antibodies IgG2bκ,IgG1κ,IgG2bκ,IgG1κ,IgG1κ,IgG1κ,IgG1κ,IgG1κ,IgG1κ,IgG1κ,IgG1κ and IgG1κ.ELISA titers of the acsites were 106-109.Indirect immunofluorescence assay (IFA)showed that the twelve mAbs could combine with the natural CDV specifically.

分类号： s851.2