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Reduction of Infectious Bursal Disease Virus Replication by shRNAs Targeting the VP1 and VP2 Genes Driven by Chicken U6 Promoter

文献类型： 会议论文

第一作者： Ouyang Wei

作者： Ouyang Wei 1 ; Ma Jinrong 2 ; Wang Yongqiang 1 ; Qin Liting 2 ; Jiang Jieyuan 3 ; Fan Hongjie 3 ; Wang Xiaomei 1 ; Wang Yongshan 2 ;

作者机构： 1.Key Laboratory of Veterinary Biological Engineering and Technology,Ministry of Agriculture

2.Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China

3.State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China

会议名称： International congress on animal hygiene

主办单位：

页码： 542-542

摘要： Infectious bursal disease virus (IBDV)causes a highly contagious and immunosuppressive disease in young chickens and results in considerable economic losses for the poultry industry.To suppress the replication of IBDV.two short hairpin RNAs (shRNAs)were designed for targeting the VPI and VP2 genes of IBDV.Recombinant plasmids carrying each shRNA or two shRNAs were constructed based on vector pSilencer2.1-U6 in which the human U6 promoter was replaced with chicken U6 promoter.In chicken embryo fibroblasts,transfection with these shRNA plasmids 24 h before infection with IBDV B87 reduced 50％ tissue culture infectious doses (TCID50)from by 108.75 TCID50/0.1 mL to 103.75-101.0 TCID50/0.1 mL.

分类号： s851.2

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