Virus-Iike Particles of Hepatitis B Virus Core Protein Containing Five Mimotopes of Infectious Bursal Disease Virus (IBDV)Protect Chickens Against IBDV
文献类型： 会议论文
第一作者： Wang Yongshan
作者： Wang Yongshan 1 ; Ouyang Wei 1 ; Liu Xiaojuan 1 ; Zhang Haibin 2 ;
作者机构： 1.Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China
2.College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China
会议名称： International congress on animal hygiene
主办单位：
页码： 492-494
摘要： Current infectic,us bursal disease virus (IBDV)vaccines suffer from maternal antibody interference and mimotope vaccines might be an alternative.Previously we demonstrated an IBDV VP2 five-mimotope polypeptide,5EPIS.elicited protective immunity in chickens.In the current study,the Sepis gene was inserted into a plasmid carrying human hepatitis B virus core protein (HBc)gene at its major immunodominant region site.The recombinant gene was efficiently expressed in E.coli to produce chimeric protein HBc-5EPIS which self-assembles to virus-like particles (VLP).Two-week old specific-pathogen-free chickens were nnmunized intramuscularly with HBc-5EPIS VLP or SEPIS polypeptide without adjuvant (50 μg/injection)on day 0,7,14 and 21.Anti-5EPIS antibody was first detected on day 7 and day 21 in HBc-5EPIS and 5EPIS group,respectively; on day 28,anti-5EPIS titers reached 12800 or 1600 by ELISA.and 3200 or 800 by virus neutralization assay in HBc-5 EPIS and 5EPIS group,respectively.No anti-5EPIS antibody was detected in the buffer control group throughout the experiment.Challenge on day 28 with a virulent IBDV strain resulted in 100％,40.0％ and 26.7％ survival for chickens immunized with HBc-5EPIS,5EPIS and buffer,respectively.These data suggest epitope presentation on chimeric VLP might be a promising approach for improving mimotope vaccines for IBDV.
分类号： s851.2
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