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Cloning of Disease Resistance-relevant Nramp and MHC IIB Genes and their Application Potential in Molecular Breeding for Disease Resistance in Japanese Flounder (Paralichthys olivaceus)

文献类型: 会议论文

第一作者: S.L. Chen

作者: S.L. Chen 1 ; Y.X. Zhang; Z.J. Wang; Z.X. Sha; M.Y. Xu; Chinese Academy of Fishery Sciences; Chinese Academy of Sciences;;

作者机构: 1.Key Lab for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 266071, Qingdao, China

关键词: MHC class IIB;nramp, cDNA;polymorphism;disease resistance;disease susceptibility;Japanese flounder, paralichthys olivaceus

会议名称: Genes,Gene Families and Isozymes

主办单位:

页码: 133-141

摘要: Nramp and MHC IIB genes were isolated from Japanese flounder (Paralichthys olivaceus) using PCR and RACE-PCR. RT-PCR indicated that Nramp transcripts were highly abundant in spleen, headkidney, abundant in intestine, liver and gill, and less abundant in heart. Significant association between MHC IIB polymorphism and diseases resistance / susceptibility was found in Japanese flounder. 13 different MHC IIB alleles were identified among 411 clones from 84 individuals. Most alleles such as allele a, b, c, d, e, f, j, k, i, m were commonly found in both resistance stock and the susceptibility stock. However, the allele g and h were observed only in resistance stock, and allele l was only found in susceptibility stock.

分类号: Q78

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