文献类型： 会议论文
第一作者： L. Zeng
作者： L. Zeng 1 ; L. Ye; V. Nerurkar; Y. Lu; Chinese Academy of Fishery Sciences; Chinese Academy of Sciences;;
作者机构： 1.Yangtze River Fishery Research Institute, CAFS, Jingzhou, Hubei 434000, China
会议名称： Genes,Gene Families and Isozymes
主办单位：
页码： 195-200
摘要： We have optimized a protocol for the bulky preparation of HIV-1-based defective lentiviral vector (DLV) and the concentration of vector through one-step ultracentrifugation. DLV demonstrated high transduction efficiency in human T-cell lines. Long-term culture of transduced CEM-ss cells detected by genome DNA PCR and total RNA RT-PCR showed a stable expression of reporter green fluorescent protein (GFP) gene without apparent alternation in cellular morphology and growth kinetics. 11 cell lines were tested for the susceptibility to DLV infection. Highly efficient gene transfer was revealed in CEM-ss, MT-2, Sup-T1 and HTB-14 cells. DLV is one of the promising gene transfer vehicles in the development of human gene therapy protocols.
分类号： Q78
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