文献类型： 会议论文
第一作者： J. Bai
作者： J. Bai 1 ; D. Ma; H. Lao; Q. Jian; X. Ye; J. Luo; Chinese Academy of Fishery Sciences; Chinese Academy of Sciences;;
作者机构： 1.Pearl River Fishery Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China
关键词： acipenser sinensis;cystatin;prokaryotic expression;protease inhibitory activity
会议名称： Genes,Gene Families and Isozymes
主办单位：
页码： 153-158
摘要： Cystatin, which is widely distributed in both animal and plant tissues and body fluids, can form activity-inhibitor complexes with cysteine proteases. In order to explore the functions of fish cystatin and its potential values in fish disease prevention and cure, as well as seafood process industry, we cloned the mature peptide cDNA of Chinese sturgeon (Acipenser sinensis) cystatin by RT-PCR method. Nucleotide sequence analysis showed that the cloned cDNA encodes 112 amino acid residues of the mature cystatin peptide and which is the homolog of the mammalian cystatin Family II, cystatin C. Using prokaryotic expression vector pBV220, a cystatin E.coli expression bacteria was constructed. After temperature inducement and SDS-PAGE analysis, the recombinant expression bacteria produced a special protein about 12 kD in molecular weight. The proportion of the recombinant protein in total bacterial protein was about 25%. The inhibitory activity of the recombinant cystatin was measured by inhibiting the protease activity of papain, and the result showed the specific inhibitory activities for the recombinant cystatin was calculated to be 48U/mg, where U is defined as μg papain inhibied pre μg inhibitor.
分类号： Q78
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[1]Cloning and prokaryotic expression of Chinese sturgeons cystatin cDNA. Bai, J.,Ma, D.,Lao, H.,Jian, Q.,Ye, X.,Luo, J.. 2005
[2]环境污染对中华鲟Acipenser sinensis Gray影响的研究. 姜礼燔. 1996
[3]Evolution of MHC class I genes in two ancient fish, paddlefish (Polyodon spathula) and Chinese sturgeon (Acipenser sinensis). Wang, Dengqiang,Zhong, Lei,Gan, Xiaoni,He, Shunping,Wang, Dengqiang,Wei, Qiwei,Wang, Dengqiang. 2010
[4]Effects of River Hydrological Status on Gonadal Development of Breeding Population of Chinese Sturgeon in the Yangtze River: Inferred from a On-site Observation. Zhang, Hui,Wei, Qiwei,Du, Hao. 2013
[5]Effects of high water level on the river residence period of juvenile Chinese sturgeon Acipenser sinensis in the Yangtze River. Zhang, H.,Wei, Q. W.,Du, H.,Li, C.. 2012
[6]Molecular characterization of Chinese sturgeon gonadotropins and cellular distribution in pituitaries of mature and immature individuals. Cao, Hong,Zhou, Li,Gui, Jian-Fang,Zhang, Yan-Zhen,Wei, Qi-Wei,Chen, Xi-Hua. 2009
[7]Adaptive alterations on gill Na+, K+-ATPase activity and mitochondrion-rich cells of juvenile Acipenser sinensis acclimated to brackish water. Zhao, Feng,Wu, Beibei,Yang, Gang,Zhang, Tao,Zhuang, Ping.
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