文献类型: 会议论文
第一作者: Zuowei Zhu
作者: Zuowei Zhu 1 ; Lijuan Ren; Yanghai Xiang; Xiaorong Shen; Xu Zhang; Miaoping Zhou; Weizhong Lu;
作者机构: 1.Institute of Agrobiological Genetic and Physiology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, PR. China
关键词: RAPD marker;wheat scab;resistance;recombinant inbred hnes
会议名称: Proceedings of the International Symposium on Wheat Improvement for Scab Resistance
主办单位:
页码: 132-135
摘要: A RAPD marker analysis of resistance to Fusarium head blight caused by Fusarium graminearum in wheat Sumai 3 (highly resistance to FHB) was investigated using F_1 recombinant inbred hnes (RILs). The RILs were derived from the F, of the cross 'Sumai 3/Alondra' by single-seed descent. The resistance of RILs was evaluated in the field by injecting conidiospores into a central floret, and scab symptoms were recorded 21 days after inoculation. Disease severity was calculated as the percentage of infectedspikelets/spike. DNA from resistant and susceptible parents was analyzed with 546 RAPD primers. Ninety-three of these primers amphfied polymorphic band between parents. Four RAPD primers (S1004, S1022, S1120 and S1139) showed hnkage with the resistance to FHB, with recombination values of 18.94, 24.26, 28.30, and 24.49 %, respectively. Because these four primers were not hnked to each other, they may be linked to different resistance genes of Sumai 3.
分类号: S512
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