An asymmetric PCR-based, reliable and rapid single-tube native DNA engineering strategy

Bi, Yanzhen; Qiao, Xianfeng; Hua, Zaidong; Zhang, Liping; Liu, Ximei; Li, Li; Hua, Wenjun; Xiao, Hongwei; Zhou, Jingrong; Wei, Qingxin; Zheng, Xinmin; SCI

机构： Hubei Acad Agr Sci, Inst Anim Sci & Vet Med, Hubei Key Lab Anim Embryo Engn & Mol Breeding, Wuhan 430064, Peoples R China; Hubei Acad Agr Sci, Inst Anim Sci & Vet ;

关键词： Asymmetric Bridge PCR Intramolecular homologous recombination Myostatin

 全文链接 请求原文

Rapid, Simple and Ultrasensitive Analysis of Paraquat in Drinking Water by Field-Amplified Sample Injection Combined with Pressure-Assisted Capillary Zone Electrophoresis

Wang Ming-Ming; Song Ting; Li Sheng-Qing; Chen Hao; Shen Jing; SCI

机构： Huazhong Agr Univ, Coll Sci, Wuhan 430070, Peoples R China; Hubei Acad Agr Sci, Inst Agr Qual Stand & Testing Technol, Wuhan 430064, Peoples R China;

关键词： Field-amplified sample injection Capillary zone electrophoresis Paraquat Drinking water

 全文链接 请求原文

Identification of genome-wide copy number variations among diverse pig breeds by array CGH

Li, Yan; Zhang, Xuying; Liu, Gang; Tao, Hu; Jiang, Siwen; Xiong, Yuanzhu; Li, Fenge; Li, Yan; Zhang, Xuying; Liu, Gang; Tao, Hu; Jiang, Siwen; Xiong, Yuanzhu; Li, Fenge; Mei, Shuqi; Peng, Xianwen; Wu, Huayu; SCI

机构： Huazhong Agr Univ, Minist Agr, Key Lab Pig Genet & Breeding, Wuhan 430070, Peoples R China; Huazhong Agr Univ, Lab Agr Anim Genet,Breeding & Reprod, Minist Educ, Wuhan 430070, Peoples R China; Huazhong Agr Univ, Lab Agr Anim Genet,Breeding & Reprod, Minist Educ, Wuhan 4;

 全文链接 请求原文

Effects of Tillage and Nitrogen Fertilizers on CH4 and CO2 Emissions and Soil Organic Carbon in Paddy Fields of Central China

Li Cheng-Fang; Kou Zhi-Kui; Zhang Zhi-Sheng; Wang Jin-Ping; Cai Ming-Li; Cao Cou-Gui; Zhou Dan-Na; SCI

机构： Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan, Hubei, Peoples R China; Hubei Acad Agr Sci, Inst Anim Husb & Vet Sci, Wuhan, Hubei, Peoples R China;

 全文链接 请求原文

Molecular characterization, expression pattern, and association analysis with carcass traits of the porcine SHIP2 gene

Xiong, Qi; Li, Xiaofeng; Suo, Xiaojun; Zhang, Nian; Yang, Qianping; Liu, Yang; Chen, Mingxin; Chai, Jin; Deng, Changyan; Jiang, Siwen; Zheng, Rong; Chai, Jin; Deng, Changyan; Jiang, Siwen; Zheng, Rong; SCI

机构： Hubei Acad Agr Sci, Inst Anim Husb & Vet, Hubei Key Lab Anim Embryo Engn & Mol Breeding, Wuhan 430064, Peoples R China; Hubei Acad Agr Sci, Agr Minist Key Lab Swine Breeding & Genet, Coll Anim Sci & Technol, Wuhan 430064, Peoples R China; Hubei Acad Agr Sci, Agr Minist Key Lab Swine Breeding & Genet, Co;

关键词： SHIP2 E2F MyoD Carcass traits Pig Promoter analysis

 全文链接 请求原文

Runoff and nutrient losses in citrus orchards on sloping land subjected to different surface mulching practices in the Danjiangkou Reservoir area of China

Liu, Y.; Tao, Y.; Wan, K. Y.; Zhang, G. S.; Chen, F.; Liu, D. B.; Xiong, G. Y.; SCI

机构： Chinese Acad Sci China, Lab Aquat Bot & Watershed Ecol, Wuhan 430074, Peoples R China; Hubei Acad Agr Sci, Inst Plant Protect & Soil Fertilizer, Wuhan 430064, Peoples R China;

关键词： Water and soil losses Sediment yield Straw mulching Plastic film mulching

 全文链接 请求原文

Long-term pig manure application reduces the requirement of chemical phosphorus and potassium in two rice-wheat sites in subtropical China

Xu, M.; He, X.; He, X.; He, X.; He, X.; Li, S.; Sun, X.; SCI

机构： Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Minist Agr China, Key Lab Crop Nutr & Fertilizat, Beijing 100081, Peoples R China; Edith Cowan Univ, Sch Nat Resources, Ctr Ecosyst Management, Joondalup, WA 6027, Australia; Univ Western Australia, State Ctr Excellence Ecohydrol, Crawley, WA 6009, Australia; Univ Western Australia, Sch Plant Biol, Crawley, WA 6009, Australia; Hubei Acad Agr Sci, Inst Plant Protect & Soil Sci, Wuhan 430064, Peoples R China; Sichuan Acad Agr Sci, Soil & Fertilizer Inst, Chengdu 610066, Peoples R China;

关键词： Nitrogen loss nitrogen recovery efficiency manure phosphorus potassium

 在线阅读   全文下载 请求原文

Multiple amino acid variations in the nonstructural proteins of swine Japanese encephalitis virus alter its virulence in mice

Wu, Rui; Tian, Yongxiang; Deng, Junhua; Yang, Keli; Liang, Wangwang; Guo, Rui; Duan, Zhengying; Liu, Zewen; Zhou, Danna; Xu, Diping; Wu, Rui; Tian, Yongxiang; Xu, Diping; Wu, Rui; SCI

机构： Hubei Acad Agr Sci, Inst Anim Husb & Vet Sci, Wuhan 430064, Hubei, Peoples R China; Hubei Key Lab Anim Embryo Engn & Mol Breeding, Wuhan 430064, Hubei, Peoples R China; Hubei Key Lab ;

关键词： Japanese encephalitis virus Swine Mouse Adaptation Pathogenicity

 全文链接 请求原文

Effects of cyclic AMP on development and secondary metabolites of Monascus ruber M-7

Chen, F.; Chen, F.; Lai, Y.; Wang, L.; Qing, L.; Chen, F.; SCI

机构： State Key Lab Agr Microbiol, Wuhan 430070, Hubei Province, Peoples R China; Minist Agr, Key Lab Food Safety Evaluat, Wuhan, Hubei Province, Peoples R China; Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan, Hubei Province, Peoples R China;

关键词： cAMP development fermentation metabolism Monascus ruber

 全文链接 请求原文

One-stop polymerase chain reaction (PCR): An improved PCR method with speedy operation and comparable efficiency

Bi, Yan-zhen; Zheng, Xin-min; Qiao, Xian-feng; Zhang, Jia-nong; Ou-yang, Hui-Wu; Ma, Jun; SCI

机构： Hubei Acad Agr Sci, Inst Anim Sci & Vet Med, Hubei Key Lab Anim Embryo Engn & Mol Breeding, Wuhan 430064, Peoples R China; Wuhan Univ, Coll Life Sci, RNA Grp, Wuhan 430072, Peoples R China; Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China;

关键词： Polymerase chain reaction (PCR) post-cycling compatible dye Gelred

 全文链接 请求原文