Reference gene selection for quantitative gene expression studies during biological invasions: A test on multiple genes and tissues in a model ascidian Ciona savignyi

文献类型： 外文期刊

第一作者： Huang, Xuena

作者： Huang, Xuena;Gao, Yangchun;Zhan, Aibin;Jiang, Bei;Zhou, Zunchun

作者机构：

关键词： Ascidian; Biological invasions; Ciona savignyi; Gene expression; Reference genes; qRT-PCR

期刊名称：GENE （ 影响因子：3.913； 五年影响因子：3.48 ）

ISSN： 0378-1119

年卷期： 2016 年 576 卷 1 期

页码：

收录情况： SCI

摘要： As invasive species have successfully colonized a wide range of dramatically different local environments, they offer a good opportunity to study interactions between species and rapidly changing environments. Gene expression represents one of the primary and crucial mechanisms for rapid adaptation to local environments. Here, we aim to select reference genes for quantitative gene expression analysis based on quantitative Real-Time PCR (qRT-PCR) for a model invasive ascidian, Ciona savignyi. We analyzed the stability of ten candidate reference genes in three tissues (siphon, pharynx and intestine) under two key environmental stresses (temperature and salinity) in the marine realm based on three programs (geNorm, NormFinder and delta Ct method). Our results demonstrated only minor difference for stability rankings among the three methods. The use of different single reference gene might influence the data interpretation, while multiple reference genes could minimize possible errors. Therefore, reference gene combinations were recommended for different tissues - the optimal reference gene combination for siphon was RPS15 and RPL17 under temperature stress, and RPL17, UBQ and TubA under salinity treatment; for pharynx, TubB, TubA and RPL17 were the most stable genes under temperature stress, while TubB, TubA and UBQ were the best under salinity stress; for intestine, UBQ, RPS15 and RPL17 were the most reliable reference genes under both treatments. Our results suggest that the necessity of selection and test of reference genes for different tissues under varying environmental stresses. The results obtained here are expected to reveal mechanisms of gene expression-mediated invasion success using C savignyi as a model species. (C) 2015 Elsevier B.V. All rights reserved.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序