您好,欢迎访问河南省农业科学院 机构知识库!

马立克病病毒编码的mi R-M4-5 p调控宿主靶基因的筛选及鉴定

文献类型: 中文期刊

作者: 苏芮 1 ; 滕蔓 2 ; 李会珍 1 ; 郑鹿平 2 ; 刘豪丽 2 ; 刘金玲 2 ; 朱志坚 2 ; 罗俊 2 ; 张改平 1 ;

作者机构: 1.河南农业大学牧医工程学院

2.河南省农业科学院动物免疫学重点实验室

关键词: MDV;miRNA;宿主靶基因;肿瘤发生;hybrid-PCR

期刊名称: 畜牧兽医学报

ISSN: 0366-6964

年卷期: 2020 年 51 卷 008 期

页码: 1956-1969

收录情况: 北大核心 ; CSCD

摘要: 血清1型马立克病病毒(MDV-1)感染引起的鸡马立克病(MD)一直以来被认为是研究病毒诱导肿瘤发生机制的理想模型.此前研究发现,MDV-1编码的miR-M4-5p是宿主癌基因miR-155的病毒同源物,从基因组中敲除miR-M4-5p可显著降低MDV-1的致病性和致瘤性,表明miR-M4-5p可能是MDV-1诱导肿瘤发生的重要调控因子.为进一步揭示miR-M4-5p的调控机制,以CEF细胞总RNA反转录产物cDNA为模板,利用hybrid-PCR技术构建了miR-M4-5p的候选靶基因文库.通过基因克隆、PCR鉴定及序列比对分析,获得128个候选基因序列,有73个基因的3′-UTR存在miR-M4-5p的潜在结合靶点,其中23个3′-UTR结合靶点与miR-M4-5p完全互补配对.通过双荧光素酶报告试验和qRT-PCR分析,对miR-M4-5p与3′-UTR的体内外相互作用以及候选靶基因的表达水平进行分析和验证,最终鉴定DPT、TMEM230和DCLK1为miR-M4-5p调控的宿主靶基因.本研究为后续进一步阐明miR-M4-5p在MD肿瘤发生中的调控机制奠定了重要基础.

  • 相关文献

[1]马立克病病毒miR-M4-5p宿主靶基因cDNA文库的构建及鉴定. 薛正飞,滕蔓,李会珍,马圣明,宋利娜,张雅,罗俊,张改平. 2018

[2]马立克氏病病毒编码的microRNA:从基因组学到功能研究. 罗俊,滕蔓,樊剑鸣,王方雨,周玲,邓瑞广,张改平. 2010

[3]Marek's disease virus-encoded microRNAs: genomics, expression and function. Luo Jun,Teng Man,Fan JianMing,Wang FangYu,Zhou Ling,Deng RuiGuang,Zhang GaiPing,Fan JianMing. 2010

[4]马立克病病毒分子生物学检测技术研究进展. 张文凯,董松岭,滕蔓,李桂喜,罗琴,路文渊,郑鹿平,丁轲,刘金玲,余祖华,罗俊. 2022

[5]LAT基因簇miRNA的CRISPR/Cas9基因编辑对马立克病病毒体外复制的影响分析. 楚钰淑,滕蔓,周子誉,石彬,郑鹿平,刘金玲,罗俊,张改平. 2021

[6]Marek's disease virus type 1 encoded analog of miR-155 promotes proliferation of chicken embryo fibroblast and DF-1 cells by targeting hnRNPAB. Dang, Lu,Zhou, En-Min,Zhang, Gai-Ping,Dang, Lu,Teng, Man,Li, Hui-Zhen,Ma, Sheng-Ming,Lu, Qing-Xia,Hao, Hui-Fang,Zhao, Dong,Luo, Jun,Li, Hui-Zhen,Zhang, Gai-Ping,Ma, Sheng-Ming,Luo, Jun,Zhang, Gai-Ping.

[7]Marek's disease virus-encoded analog of microRNA-155 activates the oncogene c-Myc by targeting LTBP1 and suppressing the TGF-beta signaling pathway. Chi, Jia-Qi,Yu, Zu-Hua,Chi, Jia-Qi,Teng, Man,Yu, Zu-Hua,Su, Jing-Wei,Zhao, Pu,Xing, Guang-Xu,Deng, Rui-Guang,Luo, Jun,Su, Jing-Wei,Zhao, Pu,Liang, Hong-De,Zhang, Gai-Ping,Qu, Liang-Hu,Zhang, Gai-Ping.

[8]Molecular characteristics and evolutionary analysis of field Marek's disease virus prevalent in vaccinated chicken flocks in recent years in China. Yu, Zu-Hua,Chi, Jia-Qi,Hu, Bo,Liu, Ju-Xiong,Yu, Zu-Hua,Teng, Man,Luo, Jun,Yu, Le-Le,Su, Jing-Wei,Chi, Jia-Qi,Zhao, Pu,Hu, Bo,Zhang, Gai-Ping,Yu, Zu-Hua,Ding, Ke,Luo, Jun,Wang, Xin-Wei,Su, Jing-Wei.

[9]Virus-encoded miR-155 ortholog is an important potential regulator but not essential for the development of lymphomas induced by very virulent Marek's disease virus. Yu, Zu-Hua,Teng, Man,Yu, Le-Le,Hu, Bo,Zhang, Gai-Ping,Luo, Jun,Yu, Zu-Hua,Hu, Bo,Liu, Ju-Xiong,Zhang, Gai-Ping,Sun, Ai-Jun,Cui, Zhi-Zhong,Qu, Liang-Hu,Yu, Zu-Hua,Ding, Ke,Cheng, Xiang-Chao,Luo, Jun.

[10]玉米雌穗发育杂种优势相关miRNA的研究. 王琪月,孟淑君,张柯,张战辉,汤继华,丁冬. 2018

[11]胚胎移植前供体牛与受体牛血浆外泌体miRNA差异表达分析. 翟亚莹,施巧婷,楚秋霞,朱肖亭,滑留帅,张子敬,陈付英,祁兴磊,王二耀,吕世杰. 2022

[12]乏情和发情初产母猪下丘脑-垂体轴中miRNA表达谱比较分析. 任巧玲,张家庆,王璟,陈俊峰,马强,高彬文,刘付玖,邢宝松. 2023

[13]miRNA在奶牛子宫内膜炎治疗靶点的研究进展. 张志浩,张子敬,吕世杰,朱肖亭,李欣淼,张格阳,闵佳,屈俊峰,黄永震,施巧婷,王二耀,PENG Sheng-kun. 2024

[14]利用Solexa测序技术鉴定甜橙miRNAs. 王永江,李中安,张振臣,杨方云,周彦. 2013

[15]马立克氏病病毒编码的miR-M12-5p对鸡HVCN1基因表达的靶向调控. 李会珍,滕蔓,党露,冯丽丽,马圣明,赵朴,罗俊,张改平. 2016

[16]Putative roles as oncogene or tumour suppressor of the Mid-clustered microRNAs in Gallid alphaherpesvirus 2 (GaHV2) induced Marek's disease lymphomagenesis. Teng, Man,Wu, Zi-Xiang,Wu, Run,Teng, Man,Zhao, Pu,Dang, Lu,Li, Hui-Zhen,Ma, Sheng-Ming,Luo, Jun,Yu, Zu-Hua,Ma, Sheng-Ming,Luo, Jun,Zhuang, Guo-Qing,Cui, Zhi-Zhong,Zhang, Gai-Ping,Zhang, Gai-Ping.

[17]Small RNA and Degradome Deep Sequencing Reveals Peanut MicroRNA Roles in Response to Pathogen Infection. Zhao, Chuanzhi,Xia, Han,Zhao, Shuzhen,Hou, Lei,Zhang, Ye,Li, Changsheng,Wang, Xingjun,Zhao, Chuanzhi,Xia, Han,Zhao, Shuzhen,Hou, Lei,Zhang, Ye,Li, Changsheng,Wang, Xingjun,Cao, Tingjie,Zhang, Xinyou,Yang, Yu.

作者其他论文 更多>>