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Mare-145细胞无血清培养驯化、代谢分析

文献类型: 中文期刊

作者: 冯磊 1 ; 吴培培 1 ; 褚轩 1 ; 王伟峰 1 ; 侯继波 1 ;

作者机构: 1.国家兽用生物制品工程技术研究中心

关键词: Marc-145细胞;驯化培养;无血清培养;PRRSV

期刊名称: 江苏农业科学

ISSN: 1002-1302

年卷期: 2012 年 40 卷 007 期

页码: 182-185

收录情况: 北大核心 ; CSCD

摘要: 采用逐步降低培养液中血清用量的方法对Marc-145细胞进行无血清培养驯化并考察其生长代谢、增殖PRRSV(猪繁殖及呼吸综合征病毒)的能力.结果表明,通过在细胞对数生长期更换低浓度血清的培养液或传代操作,Marc-145细胞可完全适应含5%新生牛血清的营养条件,经3次传代后,Marc-145细胞可营正常贴壁生长.然而在1%新生牛血清的营养条件下,初次培养于该条件的Marc-145细胞伪足不伸展,贴壁时间明显延长.经过在该1%血清浓度下10次传代后,Marc-145细胞可贴壁生长,维持较高的存活率,但贴壁较松.由此Marc-145细胞进一步驯化适应无血清悬浮培养条件,筛选获得能够在无血清条件下悬浮生长的Marc-145细胞,且生长状态良好.比较在不同血清浓度及不同培养方式下生长的Mare-145细胞增殖PRRSV的能力,无血清条件下悬浮生长的Mare-145细胞对PRRSV的增殖性能没有改变.

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