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Phosphoenolpyruvate carboxykinase 2 (PCK2) attenuates bovine adipocyte lipolysis through PNPLA2 activity repression

文献类型： 外文期刊

作者： Jiang, Chao 1 ; Wu, Hao 1 ; Zhang, Jiupan 3 ; Song, Yaping 1 ; Song, Xiaoyu 1 ; Jiao, Ruopu 1 ; Raza, Sayed Haidar Abbas 4 ; Pant, Sameer Dinkar 6 ; Zan, Linsen 7 ; Wei, Dawei 1 ;

作者机构： 1.Ningxia Univ, Coll Anim Sci & Technol, Yinchuan 750021, Peoples R China

2.Key Lab Ruminant Mol Cell Breeding, Yinchuan 750021, Ningxia Hui, Peoples R China

3.Ningxia Acad Agr & Forestry Sci, Inst Anim Sci, Yinchuan 750021, Peoples R China

4.Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Peoples R China

5.South China Agr Univ, Guangdong Prov Key Lab Food Qual & Safety, Guangzhou 510642, Peoples R China

6.Charles Sturt Univ, Gulbali Inst, Wagga Wagga, NSW 2678, Australia

7.Northwest A&F Univ, Coll Anim Sci & Technol, Xianyang 712100, Peoples R China

关键词： PCK2; Intramuscular fat; RNA-seq; Metabolomic; PNPLA2

期刊名称：FUNCTIONAL & INTEGRATIVE GENOMICS （ 影响因子：3.1； 五年影响因子：3.0 ）

ISSN： 1438-793X

年卷期： 2025 年 25 卷 1 期

页码：

收录情况： SCI

摘要： Marbling traits serve as a key determinant of meat quality in livestock, with interspecies genetic variations significantly influencing intramuscular fat (IMF) deposition. Through systematic analysis of public databases and cross-species RNA sequencing data from cattle, sheep, and pigs exhibiting divergent IMF levels, we identified PCK2 as a conserved regulatory factor governing lipogenesis. Functional analyses demonstrated that targeted PCK2 silencing significantly impaired cell cycle dynamics and suppressed adipocyte proliferation, which mechanistically involved transcriptional downregulation of key cell cycle regulators (CCNB1, CCND1, CCNE2) and functional inhibition of critical proliferative mediators (CDK1, CDK2, PCNA). Furthermore, PCK2 silencing impaired lipid accumulation by decreasing triglyceride synthesis and lipid droplet formation, while down-regulating adipogenic transcription factors PPAR gamma and C/EBP alpha, as well as lipid metabolism factors SCD1, ACSL1 and FABP4. Integrated multi-omics analysis revealed PCK2 modulates bovine IMF deposition through the regulation of lipolysis in adipocyte. Notably, patatin-like phospholipase domain containing 2 (PNPLA2) suppression as a novel mechanism underlying PCK2-mediated enhancement of bovine intramuscular adipocyte differentiation. These findings establish a molecular framework for understanding PCK2's regulatory role in marbling beef production.

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