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APOE mediates the coupling of myogenesis and lipid metabolism in skeletal muscle: decoding intercellular crosstalk via a cell co-culture model

文献类型： 外文期刊

作者： Song, Yaping 1 ; Zhang, Jiupan 3 ; Yang, Dongmei 1 ; Jiang, Chao 1 ; Raza, Sayed Haidar Abbas 4 ; Pant, Sameer Dinkar 5 ; Ma, Yun 1 ; Zan, Linsen 6 ; Wei, Dawei 1 ;

作者机构： 1.Ningxia Univ, Coll Anim Sci & Technol, Yinchuan 750021, Peoples R China

2.Key Lab Ruminant Mol Cell Breeding, Yinchuan 750021, Ningxia Hui Aut, Peoples R China

3.Inst Anim Sci, Ningxia Acad Agr & Forestry Sci, Yinchuan 750021, Peoples R China

4.Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Peoples R China

5.Charles Sturt Univ, Gulbali Inst, Wagga Wagga, NSW 2678, Australia

6.Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Peoples R China

关键词： APOE; Co-culture; Crosstalk; Beef quality

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2025 年 315 卷

页码：

收录情况： SCI

摘要： The growth and lipid accumulation of skeletal muscle directly affects the yield and meat quality characteristics of beef, which is the result of the interaction between skeletal muscle cells (SMCs) and intramuscular adipocytes (IMAs). Therefore, in this study, a co-culture system was used to simulate the in vivo cellular microenvironment, aiming to reveal the interaction patterns between bovine SMCs and IMAs during the proliferation and differentiation stages. The results showed that, compared with SMCs cultured alone, the presence of IMAs in the co-culture system inhibited the expression of proliferation markers such as PCNA, CDK1, CDK2, CCNA2, and CCNE2 in SMCs. This also affected the G1/S phase transition of SMCs, thereby inhibiting their proliferation rate. Meanwhile, the presence of IMAs also inhibited the expression of myogenesis markers such as MYHC, MYOD, MYOG, MYF5, and MYF6, thus hindering the myotube formation process of SMCs. Moreover, RNA-seq identified that the key genes APOE, APOD, SOX9, CAV3, SMPD3, and EDN2 that mediate the interaction between SMCs and IMAs in the co-culture system. Among that, APOE has a dual effect on myogenesis and lipid accumulation. Interfering with the expression of APOE inhibited the proliferation, differentiation and lipid accumulation of SMCs. Based on these findings, we determined that APOE is a key regulatory factor connecting muscle development and fat accumulation. As a potential intervention target, APOE has great potential and can open up new avenues for improving meat quality and production efficiency.

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