文献类型： 外文期刊
作者： Jiang, Chao 1 ; Zhang, Jiupan 3 ; Song, Yaping 1 ; Song, Xiaoyu 1 ; Wu, Hao 1 ; Jiao, Ruopu 1 ; Li, Lanlan 1 ; Zhang, Guijie 1 ; Wei, Dawei 1 ;
作者机构： 1.Ningxia Univ, Coll Anim Sci & Technol, Yinchuan 750021, Peoples R China
2.Key Lab Ruminant Mol Cell Breeding, Yinchuan 750021, Ningxia Hui Aut, Peoples R China
3.Ningxia Acad Agr & Forestry Sci, Inst Anim Sci, Yinchuan 750021, Peoples R China
关键词： Bovine; FOXO1; Myogenesis; RNA-seq; MYH3
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.2； 五年影响因子：7.8 ）
ISSN： 0141-8130
年卷期： 2024 年 260 卷
页码：
收录情况： SCI
摘要： The growth and development of bovine skeletal muscle and beef yield is closely intertwined. Our previous research found that forkhead box O1 (FOXO1) plays an important role in the regulation of beef muscle formation, but its specific mechanism is still unknown. In this study, we aimed to clarify the regulatory mechanism of FOXO1 in proliferation and differentiation of bovine skeletal muscle cells (BSMCs). The results showed that interfering with FOXO1 can promote proliferation and the cell G1/S phase of BSMCs by up-regulating the expression of PCNA, CDK1, CDK2, CCNA2, CCNB1, CCND1 and CCNE2. Besides, interfering with FOXO1 inhibited the apoptosis of BSMCs by up-regulating the expression of anti-apoptosis gene BCL2, while simultaneously down-regulating the expression of the pro-apoptosis genes BAD and BAX. Inversely, interfering with FOXO1 can promote the differentiation of BSMCs by up-regulating the expression of myogenic differentiation marker genes MYOD, MYOG, MYF5, MYF6 and MYHC. Furthermore, RNA-seq combined with western bolt, immunofluorescence and chromatin immunoprecipitation analysis showed that FOXO1 could regulate BSMCs differentiation process by influencing PI3K-Akt, Relaxin and TGF-beta signaling pathways, and target MYH3 for transcriptional inhibition. In conclusion, this study provides a basis for studying the role and molecular mechanism of FOXO1 in BSMCs.
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