您好，欢迎访问宁夏农林科学院 机构知识库！
宁夏农林科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Lysosomal signaling in adipogenesis regulation: A novel pathway to enhance marbled beef quality

文献类型： 外文期刊

作者： Song, Xiaoyu 1 ; Zhang, Jiupan 3 ; Song, Yaping 1 ; Jiang, Chao 1 ; Raza, Sayed Haidar Abbas 4 ; Pant, Sameer Dinkar 5 ; Ma, Yun 1 ; Ma, Yanfen 1 ; Zhang, Guijie 1 ; Zan, Linsen 6 ; Wei, Dawei 1 ;

作者机构： 1.Ningxia Univ, Coll Anim Sci & Technol, Yinchuan 750021, Peoples R China

2.Key Lab Ruminant Mol Cell Breeding, Yinchuan 750021, Ningxia Hui, Peoples R China

3.Ningxia Acad Agr & Forestry Sci, Inst Anim Sci, Yinchuan 750021, Peoples R China

4.Sun Yat Sen Univ, Sch Life Sci, Guangzhou 510275, Peoples R China

5.Charles Sturt Univ, Gulbali Inst, Wagga Wagga, NSW 2678, Australia

6.Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Shaanxi, Peoples R China

关键词： Bovine adipocytes; Lysosome; Co-culture; ZNF423

期刊名称：FOOD BIOSCIENCE （ 影响因子：5.9； 五年影响因子：6.1 ）

ISSN： 2212-4292

年卷期： 2025 年 68 卷

页码：

收录情况： SCI

摘要： Marbled beef, characterized by intramuscular fat (IMF) interspersed within muscle fibers, is a key determinant of meat quality. This study explores the molecular mechanisms underlying the interactions between bovine adipocytes (BAds) and bovine skeletal muscle cells (BSMCs) using a Transwell co-culture system. BAds and BSMCs were isolated and validated for purity and differentiation potential. Co-culture experiments revealed that BAds co-cultured with BSMCs exhibited significantly reduced proliferation and differentiation compared to BAds cultured alone, accompanied by the downregulation of key adipogenesis-related genes and proteins. Transcriptomic analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment identified the lysosome signaling pathway as a critical regulatory mechanism in this interaction. Activation of the lysosome signaling pathway with endoperoxide-6 (EN6) suppressed adipogenesis by reducing the phosphorylation levels of key signaling proteins. Conversely, lysosomal inhibition with chloroquine (CQ) enhanced lipid deposition by increasing the phosphorylation levels of these proteins. Furthermore, zinc finger protein 423 (ZNF423) was identified as a potential downstream effector of lysosomal signaling, suggesting its pivotal role in regulating adipogenesis. These findings provide new insights into the molecular mechanisms governing IMF deposition and highlight lysosomal signaling as a potential target for strategies aimed at improving meat quality.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：宁夏农林科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台