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Mutation, food-grade expression, and characterization of a lactonase for zearalenone degradation

文献类型： 外文期刊

作者： Qiu, Yangyu 1 ; Xu, Huidong 2 ; Ji, Qinyi 2 ; Xu, Rongrong 2 ; Zhu, Mulan 2 ; Dang, Yali 4 ; Shi, Xizhi 5 ; Zhang, Lili 6 ; Xia, Yu 1 ;

作者机构： 1.Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China

2.Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Peoples R China

3.Shandong Freda Bioeng Co Ltd, Jinan 250101, Peoples R China

4.Ningbo Univ, Coll Food & Pharmaceut Sci, State Key Lab Managing Biot & Chem Threats Qual &, Ningbo 315211, Peoples R China

5.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Peoples R China

6.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr, Key Lab Food Qual & Safety, Jiangsu Prov State Key Lab Breeding Base, Nanjing 210014, Peoples R China

关键词： Zearalenone; Enzyme mutation; Degradation; Kluyveromyces lactis; Food-grade

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：5.0； 五年影响因子：5.2 ）

ISSN： 0175-7598

年卷期： 2023 年 107 卷 16 期

页码：

收录情况： SCI

摘要： Zearalenone (ZEN) is a mycotoxin that causes serious threats to human health. People are exposed to ZEN contamination externally and internally through many ways, while environmental-friendly strategies for efficient elimination of ZEN are urgently needed worldwide. Previous studies revealed that the lactonase Zhd101 from Clonostachys rosea can hydrolyze ZEN to low toxicity compounds. In this work, the enzyme Zhd101 was conducted with combinational mutations to enhance its application properties. The optimal mutant (V153H-V158F), named Zhd101.1, was selected and introduced into the food-grade recombinant yeast strain Kluyveromyces lactis GG799(pKLAC1-Zhd101.1), followed by induced expression and secretion into the supernatant. The enzymatic properties of this mutant were extensively examined, revealing a 1.1-fold increase in specific activity, as well as improved thermostability and pH stability, compared to the wild-type enzyme. The ZEN degradation tests and the reaction parameters optimization were carried out in both solutions and the ZEN-contaminated corns, using the fermentation supernatants of the food-grade yeast strain. Results showed that the degradation rates for ZEN by fermentation supernatants reached 96.9% under optimal reaction conditions and 74.6% in corn samples, respectively. These new results are a useful reference to zearalenone biodegradation technologies and indicated that the mutant enzyme Zhd101.1 has potential to be used in food and feed industries.

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