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Mutations in troABCD against Copper Overload in a copA Mutant of Streptococcus suis

文献类型： 外文期刊

作者： Yang, Xia 2 ; Peng, Wei 2 ; Wang, Yanna 2 ; Yan, Kang 2 ; Liu, Zewen 1 ; Gao, Ting 1 ; Yang, Keli 1 ; Liu, Wei 1 ; Guo, Rui 1 ; Li, Chang 1 ; Chen, Huanchun 2 ; Tian, Yongxiang 1 ; Zhou, Danna 1 ; Bei, Weicheng 2 ; Yuan, Fangyan 1 ;

作者机构： 1.Hubei Acad Agr Sci, Inst Anim Husb & Vet Sci, Key Lab Prevent & Control Agents Anim Bacteriosis, Hubei Prov Key Lab Anim Pathogen Microbiol,Minist, Wuhan, Peoples R China

2.Huazhong Agr Univ, Coll Vet Med, State Key Lab Agr Microbiol, Wuhan, Peoples R China

3.Hubei Hongshan Lab, Wuhan, Peoples R China

4.Huazhong Agr Univ, Cooperat Innovat Ctr Sustainable Pig Prod, Wuhan, Peoples R China

5.Guangxi Yangxiang Co Ltd, Guigang, Guangxi, Peoples R China

关键词： Streptococcus suis; copper resistance; Mn uptake; CopA; TroABCD

期刊名称：APPLIED AND ENVIRONMENTAL MICROBIOLOGY （ 影响因子：5.005； 五年影响因子：5.632 ）

ISSN： 0099-2240

年卷期：

页码：

收录情况： SCI

摘要： Streptococcus suis is a major swine pathogen that is increasingly recognized as a porcine zoonotic pathogen that threatens the health of both pigs and humans. Metal homeostasis plays a critical role during the process of bacterial infection. In this study, RNA sequencing was used to identify potential candidate genes involved in the maintenance of intracellular copper homeostasis. CopA was identified as the primary copper exporter in S. suis. The copA deletion mutant strain was found to be more sensitive to copper and accumulated more intracellular copper than the wild-type (WT) parent strain. In addition, adding manganese increased the ability of S. suis to resist copper, and the manganese transporter, TroABCD, was involved in tolerance to copper. The copA deletion mutant strain accumulated less copper when supplemented with manganese. Furthermore, when cultured with copper, the double deletion mutant (Delta copA Delta troA) exhibited improved growth compared to the copA deletion mutant strain. In addition, the double deletion mutant (Delta copA Delta troA) accumulated less copper than the copA deletion mutant strain. These data were consistent with a model wherein defective TroABCD resulted in decreased cellular copper accumulation and protected the strain against copper poisoning.IMPORTANCE Metal homeostasis plays a critical role during the process of bacterial infection. We identified three important potential candidate genes involved in maintenance of intracellular copper homeostasis. CopA was demonstrated to be the main copper exporter in Streptococcus suis, and manganese increased the tolerance of S. suis to copper. The double deletion mutant (Delta copA Delta troA) improved growth ability over the copA deletion mutant strain in the presence of high concentrations of copper and accumulated less copper. These findings are consistent with a model wherein defective TroABCD resulted in decreased cellular accumulation of copper and protected the strain against copper poisoning. Metal homeostasis plays a critical role during the process of bacterial infection. We identified three important potential candidate genes involved in maintenance of intracellular copper homeostasis.

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