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Map-based cloning and characterization of YGL22, a new yellow-green leaf gene in rice (Oryza sativa)

文献类型： 外文期刊

作者： Zhu, Yu 1 ; Yan, Peiwen 1 ; Dong, Shiqing 1 ; Hu, Zejun 4 ; Wang, Ying 1 ; Yang, Jinshui 1 ; Xin, Xiaoyun 1 ; Luo, Xiaoji 1 ;

作者机构： 1.Fudan Univ, Sch Life Sci, State Key Lab Genet Engn, Shanghai 200438, Peoples R China

2.Fudan Univ, Sch Life Sci, MOE Engn Res Ctr Gene Technol, Shanghai 200438, Peoples R China

3.Jiangxi Agr Univ, Coll Agron, MOE Key Lab Crop Physiol Ecol & Genet Breeding, Nanchang 330045, Jiangxi, Peoples R China

4.Shanghai Acad Agr Sci, Inst Crop Breeding & Cultivat, Shanghai 201403, Peoples R China

期刊名称：CROP SCIENCE （ 影响因子：2.319； 五年影响因子：2.631 ）

ISSN： 0011-183X

年卷期： 2021 年 61 卷 1 期

页码：

收录情况： SCI

摘要： Leaf-color mutants have been extensively studied in rice (Oryza sativa L.) and many corresponding genes have been identified and cloned. However, the mechanism of complex leaf-color mutations requires further study. In this study, we obtained an introgression line (TIL22) from a set of introgression lines raised using African cultivated rice (O. glaberrima Steud.) as the donor parent and O. sativa subsp. indica Teqing as the recipient. Compared with Teqing, TIL22 showed a yellow-green leaf phenotype at the seedling stage, but the leaves gradually changed to green after the five-leaf stage. The photosynthetic pigment contents of yellow-green leaves at the seedling stage were significantly reduced and chloroplast development was retarded compared with those of Teqing. Genetic analysis indicated that the introgression line phenotype was controlled by a single nuclear gene, temporarily designated YGL22. Map-based cloning and sequence analysis suggested that the candidate gene was likely to be LOC_Os01g15390, which encodes a chloroplast protein. Real-time quantitative polymerase chain reaction (qPCR) analysis revealed that the YGL22 transcript level was significantly lower in TIL22 than that of Teqing at the seedling stage. We generated the ygl22 knockout mutant using the CRISPR/Cas9 system. The ygl22 mutants showed a similar phenotype to that of TIL22 at the seedling stage, suggesting that YGL22 may be involved in the early development of chloroplasts in rice. In addition, plant height, number of panicles per plant, and grain yield per plant of ygl22 were not significantly affected. The results indicate that YGL22 can be used as a trait marker gene in hybrid rice production.

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