您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Development and evaluation of a monoclonal antibody-based competitive ELISA for the detection of antibodies against H7 avian influenza virus

文献类型： 外文期刊

作者： Li, Yuan 1 ; Ye, Hongliu 1 ; Liu, Meng 1 ; Song, Suquan 1 ; Chen, Jin 3 ; Cheng, Wangkun 4 ; Yan, Liping 1 ;

作者机构： 1.Nanjing Agr Univ, MOE Joint Int Res Lab Anim Hlth & Food Safety, Jiangsu Engn Lab Anim Immunol, Jiangsu Detect Ctr Terr Wildlife Dis,Inst Immunol, Nanjing 210095, Jiangsu, Peoples R China

2.Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Jiangsu, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Vet Immunol & Engn, Nanjing 210014, Jiangsu, Peoples R China

4.Nanjing Hongshan Forest Zoo, Nanjing 210000, Jiangsu, Peoples R China

关键词： Avian influenza virus; H7 subtype; Competitive ELISA; Antibody detection

期刊名称：BMC VETERINARY RESEARCH （ 影响因子：2.741； 五年影响因子：2.955 ）

ISSN：

年卷期： 2021 年 17 卷 1 期

页码：

收录情况： SCI

摘要： Background: H7 subtype avian influenza has caused great concern in the global poultry industry and public health. The conventional serological subtype-specific diagnostics is implemented by hemagglutination inhibition (HI) assay despite lengthy operation time. In this study, an efficient, rapid and high-throughput competitive enzyme-linked immunosorbent assay (cELISA) was developed for detection of antibodies against H7 avian influenza virus (AIV) based on a novel monoclonal antibody specific to the hemagglutinin (HA) protein of H7 AIV. Results: The reaction parameters including antigen coating concentration, monoclonal antibody concentration and serum dilution ratio were optimized for H7 antibody detection. The specificity of the cELISA was tested using antisera against H1 similar to H9, H11 similar to H14 AIVs and other avian viruses. The selected cut-off values of inhibition rates for chicken, duck and peacock sera were 30.11, 26.85 and 45.66% by receiver-operating characteristic (ROC) curve analysis, respectively. With HI test as the reference method, the minimum detection limits for chicken, duck and peacock positive serum reached 2(0), 2(1) and 2(- 1) HI titer, respectively. Compared to HI test, the diagnostic accuracy reached 100, 98.6, and 99.3% for chicken, duck and peacock by testing a total of 400 clinical serum samples, respectively. Conclusions: In summary, the cELISA assay developed in this study provided a reliable, specific, sensitive and species-independent serological technique for rapid detection of H7 antibody, which was applicable for large-scale serological surveillance and vaccination efficacy evaluation programs.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台