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Semen extracellular vesicles mediate vertical transmission of subgroup J avian leukosis virus

文献类型： 外文期刊

作者： Liao, Liqin 1 ; Chen, Weiguo 1 ; Zhang, Xiangyu 1 ; Zhang, Huanmin 3 ; Li, Aijun 4 ; Yan, Yiming 1 ; Xie, Zi 1 ; Li, Hongxing 1 ; Lin, Wencheng 1 ; Ma, Jingyun 1 ; Zhang, Xinheng 1 ; Xie, Qingmei 1 ;

作者机构： 1.South China Agr Univ, Coll Anim Sci, Heyuan Branch, Guangdong Prov Lab Lingnan Modern Agr Sci & Techn, Guangzhou 510642, Peoples R China

2.Guangdong Prov Key Lab Agro Anim Genom & Mol Bree, Guangzhou 510642, Peoples R China

3.USDA ARS, Avian Dis & Oncol Lab, E Lansing, MI 48823 USA

4.Jinan Univ, Coll Sci & Engn, Guangzhou 510632, Peoples R China

5.Key Lab Anim Hlth Aquaculture & Environm Control, Guangzhou 510642, Guangdong, Peoples R China

6.Guangdong Engn Res Ctr Vector Vaccine Anim Virus, Guangzhou 510642, Peoples R China

关键词： Semen extracellular vesicles (SE); Subgroup J avian leukosis virus (ALV-J); Infection; Vertical transmission

期刊名称：VIROLOGICA SINICA （ 影响因子：6.947； 五年影响因子：5.997 ）

ISSN： 1674-0769

年卷期： 2022 年 37 卷 2 期

页码：

收录情况： SCI

摘要： Subgroup J avian leukosis virus (ALV-J) is a highly oncogenic retrovirus that has been devastating the global poultry industry since the late 1990s. The major infection model of ALV-J is vertical transmission, which is responsible for the congenital infection of progeny from generation to generation. Increasing evidence has suggested that extracellular vesicles (EVs) derived from virus-infected cells or biological fluids have been thought to be vehicles of transmission for viruses. However, the role of EVs in infection and transmission of ALV-J remains obscure. In the present study, semen extracellular vesicles (SE) were isolated and purified from ALV-J-infected rooster seminal plasma (SE-ALV-J), which was shown to contain ALV-J genomic RNA and partial viral proteins, as determined by RNA sequencing, reverse transcription-quantitative PCR and Western blotting. Furthermore, SE-ALV-J was proved to be able to transmit ALV-J infection to host cells and establish productive infection. More importantly, artificial insemination experiments showed that SE-ALV-J transmitted ALV-J infection to SPF hens, and subsequently mediated vertical transmission of ALV-J from the SPF hens to the progeny chicks. Taken together, the results of the present study suggested that ALV-J utilized host semen extracellular vesicles as a novel means for vertical transmission, enhancing our understanding on mechanisms underlying ALV-J transmission.

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