文献类型： 外文期刊
作者： Wang, Xingwang 1 ; Shi, Junsong 3 ; Cai, Gengyuan 1 ; Zheng, Enqin 1 ; Liu, Dewu 1 ; Wu, Zhenfang 1 ; Li, Zicong 1 ;
作者机构： 1.South China Agr Univ, Coll Anim Sci, Natl Engn Res Ctr Breeding Swine Ind, Guangzhou 510642, Guangdong, Peoples R China
2.South China Ricultural Univ, Coll Anim Sci, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou, Guangdong, Peoples R China
3.Guangdong Wens Breeding Swine Co, Yunfu, Guangdong, Peoples R China
关键词： MBD3; pig; SCNT; reprogramming
期刊名称：CELLULAR REPROGRAMMING （ 影响因子：1.987； 五年影响因子：1.887 ）
ISSN： 2152-4971
年卷期： 2019 年 21 卷 5 期
页码：
收录情况： SCI
摘要： Methyl-CpG-binding domain protein 3 (MBD3) is a core component of the nucleosome remodeling and deacetylase (NuRD) complex, which is crucial for pluripotent stem cell differentiation and embryonic development. MBD3 was shown to play important roles in transcription factor-induced somatic cell reprogramming. Expression level of MBD3 was demonstrated to be higher in somatic cell nuclear transfer-generated cloned pig embryos than in fertilization-derived porcine embryos. However, the functions of MBD3 in nuclear transfer-mediated somatic cell reprogramming are unknown. In this study, MBD3 was overexpressed in cloned pig embryos, and the effects of MBD3 overexpression on gene transcription, DNA methylation, and in vitro developmental competence of cloned pig embryos were analyzed. Results indicated that overexpression of MBD3 in cloned pig embryos not only increased blastocyst rate and number of cells per blastocyst but also upregulated mRNA expression levels and decreased the DNA methylation of NANOG, OCT4, and LINE1 genes to the levels close to those in in vivo fertilization-produced pig embryos. These findings suggest that overexpression of MBD3 improves reprogramming of cloned pig embryos.
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