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Development of a multiplex PCR to detect and discriminate porcine circoviruses in clinical specimens

文献类型： 外文期刊

作者： Yang, Keli 1 ; Jiao, Zuwu 1 ; Zhou, Danna 1 ; Guo, Rui 1 ; Duan, Zhengying 1 ; Tian, Yongxiang 1 ;

作者机构： 1.Hubei Acad Agr Sci, Inst Anim Husb & Vet, Wuhan 430064, Hubei, Peoples R China

2.Minist Agr, Key Lab Prevent & Control Agents Anim Bacteriosis, Wuhan 430064, Hubei, Peoples R China

3.Hubei Key Lab Anim Embryo & Mol Breeding, Wuhan 430064, Hubei, Peoples R China

关键词： Porcine circovirus; PCV1; PCV2; PCV3; Viral discrimination; Multiplex PCR

期刊名称：BMC INFECTIOUS DISEASES （ 影响因子：3.09； 五年影响因子：3.401 ）

ISSN：

年卷期： 2019 年 19 卷 1 期

页码：

收录情况： SCI

摘要： Background A diagnostic method to simultaneously detect and discriminate porcine circovirus type 1 (PCV1), porcine circovirus type 2 (PCV2) and porcine circovirus type 3 (PCV3) in clinical specimens is imperative for the differential diagnosis and monitoring and control of PCVs in the field. Methods Three primer pairs were designed and used to develop a multiplex PCR assay. And 286 samples from 8 farms in Hubei province were tested by the developed multiplex PCR assay to demonstrate the accuracy. Results Each of target genes of PCV1, PCV2 and PCV3 was amplified using the designed primers, while no other porcine viruses genes were detected. The limit of detection of the assay was 10 copies/mu L of PCV1, PCV2 OR PCV3. The results of the tissue samples detection showed that PCV1, PCV2 and PCV3 are co-circulating in central China. The PCV1, PCV2 and PCV3 singular infection rate was 52.4% (150/286), 61.2% (175/286) and 45.1% (129/286), respectively, while the PCV1 and PCV2 co-infection rate was 11.2% (32/286), the PCV1 and PCV3 co-infection rate was 5.9% (17/286), the PCV2 and PCV3 co-infection rate was 23.4% (67/286), and the PCV1, PCV2 and PCV3 co-infection rate was 1.7% (5/286), respectively, which were 100% consistent with the sequencing method and real-time PCR methods. Conclusions The multiplex PCR assay could be used as a differential diagnostic tool for monitoring and control of PCVs in the field. The results also indicate that the PCVs infection and their co-infection are severe in Hubei province, Central China.

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