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Development of an immunochromatographic strip test for the rapid detection of soybean Bowman-Birk inhibitor

文献类型： 外文期刊

作者： Wang, Yao 1 ; Li, Yanhong 1 ; Wu, Jiabei 1 ; Pei, Yafeng 1 ; Chen, Xiujin 1 ; Sun, Yaning 2 ; Hu, Mei 2 ; Xing, Yunrui 2 ; C 1 ;

作者机构： 1.Henan Univ Sci & Technol, Coll Food & Bioengn, Luoyang 471023, Henan, Peoples R China

2.Henan Acad Agr Sci, Minist Agr, Henan Key Lab Anim Immunol, Key Lab Anim Immunol, Zhengzhou, Henan, Peoples R China

3.Henan Univ Sci & Technol, Natl Demonstrat Ctr Expt Food Proc & Safety Educ, Luoyang 471023, Henan, Peoples R China

关键词： Bowman-Birk inhibitor; monoclonal antibody; immunochromatographic strip

期刊名称：FOOD AND AGRICULTURAL IMMUNOLOGY （ 影响因子：3.101； 五年影响因子：2.573 ）

ISSN： 0954-0105

年卷期： 2019 年 30 卷 1 期

页码：

收录情况： SCI

摘要： Bowman-Birk inhibitor (BBI) is the main type of soybean trypsin inhibitor, which is a kind of antinutritional factor. It can cause indigestion and hinder growth and development of human and animal. Herein, a rapid immunochromatographic strip was developed in a sandwich format using the anti-BBI monoclonal antibody (mAb) in order to specifically detect BBI. The mAbs showed high affinity towards BBI and no cross-reactivity with other soybean allergenic proteins and antinutritional factors. The assay could be performed within 10?min. The limit of detection of the test strip was 0.5??g/mL for visual and 0.23??g/mL for strip reader. The assay showed high specificity for BBI and recovery efficacy of BBI in spiked milk samples ranged between 83.24% and 89.86% with the coefficient of variation of less than 7.98%. Therefore, the test strip could be used as a rapid and reliable detection method for BBI qualitative or quantitative assessment.

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