文献类型： 外文期刊
作者： Han, Xiaojing 1 ; Zhai, Zhiyao 1 ; Yang, Xiaopeng 1 ; Zhang, Di 3 ; Tang, Jun 1 ; Zhu, Jianming 1 ; Zhu, Xiaofei 1 ; Ye, 1 ;
作者机构： 1.Zhengzhou Univ, Green Catalysis Ctr, Zhengzhou 450001, Peoples R China
2.Zhengzhou Univ, Coll Chem, Zhengzhou 450001, Peoples R China
3.Henan Acad Agr Sci, Inst Agr Qual Stand & Testing Technol, Zhengzhou 450002, Peoples R China
4.Zhengzhou Univ, Journal Zhengzhou Univ, Zhengzhou, Peoples R China
期刊名称：ORGANIC & BIOMOLECULAR CHEMISTRY （ 影响因子：3.876； 五年影响因子：3.397 ）
ISSN： 1477-0520
年卷期： 2020 年 18 卷 7 期
页码：
收录情况： SCI
摘要： As an important biothiol in living cells, cysteine is closely related to oxidative damage in living organisms. Sulfite from cysteine metabolism in living cells plays a crucial role in maintaining homeostasis in an organism, and the unbalance of sulfite in vivo would lead to multiple diseases. Thus the development of a new fluorescent probe for cysteine metabolism is needed urgently in mitochondria which are the main place of cysteine metabolism. Herein we construct a novel targeting mitochondria fluorescent probe CP-K based on the FRET mechanism to visualize sulfite in living MCF-7 cells. Probe CP-K displays a large Stokes shift of 150 nm, a low detection limit (26.3 nM) and "naked eye" detection after the addition of HSO3-. Importantly, it is appropriate for imaging the endogenous sulfite from cysteine metabolism in living cells.
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