Source and Follicular Fluid Treatment During the In Vitro Maturation of Recipient Oocytes Affects the Development of Cloned Pig Embryo
文献类型： 外文期刊
作者： Zhao, Huaxing 1 ; Xie, Shaoyi 1 ; Zhang, Ning 1 ; Ao, Zheng 3 ; Wu, Xiao 1 ; Yang, Liusong 1 ; Shi, Junsong 4 ; Mai, Ranb 1 ;
作者机构： 1.South China Agr Univ, Coll Anim Sci, Natl Engn Res Ctr Breeding Swine Ind, Guangzhou 510642, Peoples R China
2.South China Agr Univ, Coll Anim Sci, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou, Peoples R China
3.Guizhou Univ, Coll Anim Sci, Minist Educ, Key Lab Anim Genet Breeding & Reprod Plateau Mt R, Guiyang, Peoples R China
4.Wens Foodstuff Grp Co Ltd, Guangdong Wens Pig Breeding Technol Co Ltd, Yunfu, Peoples R China
关键词： pig; recipient oocytes; IVM; follicular fluid; SCNT embryos
期刊名称：CELLULAR REPROGRAMMING （ 影响因子：1.987； 五年影响因子：1.887 ）
ISSN： 2152-4971
年卷期： 2020 年 22 卷 2 期
页码：
收录情况： SCI
摘要： Pig cloning technique is valuable in agriculture, biomedicine, and life sciences. However, the full-term developmental efficiency of cloned pig embryos is only about 1%, which limits pig cloning application. The quality of recipient oocytes greatly affects the developmental competence of cloned pig embryos. Thus, this study investigated the effects of a recipient oocyte source (in vivo matured [IVVM] oocytes vs. slaughter house-derived in vitro matured [IVTM] oocytes), and follicular liquid treatment (slaughter house-derived immature follicle-derived fluid [IFF] vs. in vivo-matured follicle-derived fluid [MFF]) during the in vitro maturation (IVM) of oocytes on the development of the cloned pig embryos. Our results showed that using IVVM oocytes to replace IVTM oocytes as recipient oocytes, and using 10% MFF IVM medium to replace 10% IFF IVM medium could enhance the development of the cloned pig embryos. IFF and MFF contained different levels of oocyte quality-related proteins, resulting in different oocyte quality-related gene expression levels and reactive oxygen species levels between the 10% MFF medium-cultured oocytes and 10% IFF medium-cultured oocytes. This study provided useful information for enhancing the pig cloning efficiency by improving the quality of recipient oocytes.
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