Genetic dissection of Meloidogyne incognita resistance genes based on VIGS functional analysis in Cucumis metuliferus
文献类型： 外文期刊
作者： Xie, Xiaoxiao 1 ; Ling, Jian 1 ; Lu, Junru 1 ; Mao, Zhenchuan 1 ; Zhao, Jianlong 1 ; Zheng, Shijie 1 ; Yang, Qihong 1 ; Li, Yan 1 ; Visser, Richard G. F. 2 ; Bai, Yuling 2 ; Xie, Bingyan 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, State Key Lab Vegetable Biobreeding, Beijing 100081, Peoples R China
2.Wageningen Univ & Res, Plant Breeding, Droevendaalsesteeg 1, NL-6708 PB Wageningen, Netherlands
3.Wageningen Univ & Res, Grad Sch Expt Plant Sci, Wageningen, Netherlands
4.Shanghai Acad Agr Sci, Shanghai Key Lab Protected Hort Technol, Shanghai 201403, Peoples R China
5.Beijing Normal Univ, Coll Life Sci, Beijing 100875, Peoples R China
关键词： Cucumis metuliferus; Meloidogyne incognita; Bin map; QTL; VIGS
期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.8； 五年影响因子：5.4 ）
ISSN： 1471-2229
年卷期： 2024 年 24 卷 1 期
页码：
收录情况： SCI
摘要： The southern root-knot nematode, Meloidogyne incognita, is a highly serious plant parasitic nematode species that causes significant economic losses in various crops, including cucumber (Cucumis sativus L.). Currently, there are no commercial cultivars available with resistance to M. incognita in cucumber. However, the African horned melon (Cucumis metuliferus Naud.), a semi-wild relative of cucumber, has shown high resistance to M. incognita. In this study, we constructed an ultrahigh-density genetic linkage bin-map using low-coverage sequences from an F2 population generated through the cross between C. metuliferus inbred lines CM3 and CM27. Finally, we identified a QTL (quantitative trait locus, QTL3.1) with a LOD (logarithm of the odds) score of 3.84, explaining 8.4% of the resistance variation. Subsequently, by combining the results of qPCR (quantitative PCR) and VIGS (virus-induced gene silencing), we identified two genes, EVM0025394 and EVM0006042, that are potentially involved in the resistance to M. incognita in CM3. The identification of QTLs and candidate genes in this study serve as a basis for further functional analysis and lay the groundwork for harnessing this resistance trait.
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