Irreversible coumarin based fluorescent probe for selective detection of Cu2+ in living cells
文献类型： 外文期刊
作者： Ahmed, Nadeem 1 ; Zareen, Wajeeha 1 ; Zhang, Di 3 ; Yang, Xiaopeng 1 ; Ye, Yong 1 ;
作者机构： 1.Zhengzhou Univ, Green Catalysis Ctr, Zhengzhou 450001, Peoples R China
2.Zhengzhou Univ, Coll Chem, Zhengzhou 450001, Peoples R China
3.Henan Acad Agr Sci, Inst Agr Qual Stand & Testing Technol, Zhengzhou 450002, Peoples R China
关键词： Fluorescent probe; Copper ions; KYSE30 cells; Catalytic hydrolysis; Fluorescence imaging
期刊名称：SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY （ 影响因子：4.831； 五年影响因子：4.073 ）
ISSN： 1386-1425
年卷期： 2022 年 264 卷
页码：
收录情况： SCI
摘要： Copper ion (Cu2+) is an essential part of the living organisms. Cu2+ ions play a vital role in many biotic processes. An abnormal amount of Cu2+ ions may result in serious diseases. Herein, a novel "fluorescent ON" probe NC-Cu to trace minute levels of Cu2+ ions in presence of various biological active species has been developed. Lysosomal cells targeting group (Morpholine) was added to the probe. The spectral properties of probe NC-Cu were recorded in HEPES buffer (0.01 M, pH = 7.4, comprising 50% CH3CN, lambda(ex) = 430-nm, slit: 5 nm). The synthesized probe NC-Cu work based on copper promoted catalytic hydrolysis of hydrazone and shows remarkable fluorescence enhancement. The reaction of the probe with Cu2+ ions was completed within 20 min. An excellent linear relationship (R-2 = 0.9952) was found and the limit of detection (LOD, according to the 3 sigma/slope) for Cu2+ ions was calculated to be 5.8 mu M. Furthermore, NC-Cu was effectively functional in the living cells (KYSE30 cells) to trace Cu2+ ions. (C) 2021 Elsevier B.V. All rights reserved.
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