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Molecular cloning, expression analysis of the IgT gene and detection of IgT(+) B cells in the half-smooth tongue sole (Cynoglossus semilaevis)

文献类型： 外文期刊

作者： Wang, Lei 1 ; Zhang, Fumiao 3 ; Chen, Yadong 1 ; Wang, Shuangyan 1 ; Chen, Zhangfan 1 ; Zhou, Qian 1 ; Chen, Songlin 1 ;

作者机构： 1.Chinese Acad Fishery Sci CAFS, Key Lab Sustainable Dev Marine Fisheries, Minist Agr, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

2.Pilot Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China

3.Shandong Normal Univ, Jinan 250014, Peoples R China

关键词： IgT; Half-smooth tongue sole; Mucosal related immune response; Immunofluorescence analysis; IgT(+) B lymphocyte Cell

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.622； 五年影响因子：4.799 ）

ISSN： 1050-4648

年卷期： 2022 年 123 卷

页码：

收录情况： SCI

摘要： IgT is a specific Ig isotype in teleosts, which plays extremely important roles in the mucosal immunity of fish. In the present study, the membrane-bound and secretory IgT of the half-smooth tongue sole (Cynoglossus semilaevis) were identified for the first time. The V-D-J-C structure of two forms of csIgT are translated by the same C tau gene, and the secretory tail and transmembrane domain were encoded through alternative splicing at the 3 & PRIME; end of the C tau 4. The CH regions of csIgT had high similarity with that of other flatfish (P. olivaceus and S. maximus). In healthy C. semilaevis, sIgT and mIgT were mainly expressed in mucus related tissues such as skin, intestine and gill. The transcript levels of sIgT and mIgT mRNA showed a significant induction in the immune-related tissues upon Vibrio Harveyi infection. A polyclonal rabbit anti-csIgT was successfully prepared using the csIgT heavy chain recombinant protein. Using this antibody, we detected the native IgT with the molecular mass at 220 kDa in skin total protein under non-reducing SDS-PAGE condition. Immunofluorescence analysis indicated that IgT(+) B lymphocytes were intensively located in the skin, gill, intestine, and head kidney of C. semilaevis. These results suggest that IgT may participate in the immune response of C. semilaevis, which will facilitate the investigations of the immunoglobulins of marine fish.

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